PCR设计引物时酶切位点的保护碱基表.doc

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1、PCR设计引物时酶切位点的保护碱基表1酶寡核苷酸序列切割率%2hr20hrAccIGGTCGACCCGGTCGACCGCCGGTCGACCGG000000AflIIICACATGTGCCACATGTGGCCCACATGTGGG0>90>900>90>90AscIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA>90>90>90>90>90>90AvaICCCCGGGGCCCCCGGGGGTCCCCCGGGGGA50>90>90>90>90>90PCR设计引物时酶切位点的保护碱基表2酶寡核苷酸序列切割率%2hr20hrBamHICGGATCCGCGGGATCCCGCGCGGATCC

2、GCG10>90>9025>90>90BglIICAGATCTGGAAGATCTTCGGAAGATCTTCC075250>90>90BssHIIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA005000>90BstEIIGGGT(A/T)ACCC010BstXIAACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT02525050>90PCR设计引物时酶切位点的保护碱基表3酶寡核苷酸序列切割率%2hr20hrClaICATCGATGGATCGATCCCATCGATGGCCCATCGATGG

3、G00>905000>9050EcoRIGGAATTCCCGGAATTCCGCCGGAATTCCGG>90>90>90>90>90>90HaeIIIGGGGCCCCAGCGGCCGCTTTGCGGCCGCAA>90>90>90>90>90>90HindIIICAAGCTTGCCAAGCTTGGCCCAAGCTTGGG00100075KpnIGGGTACCCGGGGTACCCCCGGGGTACCCCG0>90>900>90>90PCR设计引物时酶切位点的保护碱基表4酶寡核苷酸序列切割率%2hr20hrMluIGACGCGTCCGACGCGTCG025050NcoICCCATGGGCATGCCA

4、TGGCATG050075NdeICCATATGGCCCATATGGGCGCCATATGGCGGGGTTTCATATGAAACCCGGAATTCCATATGGAATTCCGGGAATTCCATATGGAATTCCC000075750000>90>90NheIGGCTAGCCCGGCTAGCCGCTAGCTAGCTAG0101002550PCR设计引物时酶切位点的保护碱基表5酶寡核苷酸序列切割率%2hr20hrNotITTGCGGCCGCAAATTTGCGGCCGCTTTAAAATATGCGGCCGCTATAAAATAAGAATGCGGCCGCTAAACTATAAGGAAAAAAGCGGCCG

5、CAAAAGGAAAA0101025250101090>90NsiITGCATGCATGCACCAATGCATTGGTTCTGCAGTT10>90>90>90PacITTAATTAAGTTAATTAACCCTTAATTAAGG000025>90PmeIGTTTAAACGGTTTAAACCGGGTTTAAACCCAGCTTTGTTTAAACGGCGCGCCGG0007502550>90PstIGCTGCAGCTGCACTGCAGTGCAAACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT010>90>9

6、00010>90>900酶切位点保护碱基表6酶寡核苷酸序列切割率%2hr20hrPvuICCGATCGGATCGATCGATTCGCGATCGCGA010002510SacICGAGCTCG1010SacIIGCCGCGGCTCCCCGCGGGGA0500>90SalIGTCGACGTCAAAAGGCCATAGCGGCCGCGCGTCGACGTCTTGGCCATAGCGGCCGCGGACGCGTCGACGTCGGCCATAGCGGCCGCGGAA0101005075ScaIGAGTACTCAAAAGTACTTTT10752575SmaICCCGGGCCCCGGGGCCCCCGGGGGTCCC

7、CCGGGGGA0010>90101050>90SpeIGACTAGTCGGACTAGTCCCGGACTAGTCCGCTAGACTAGTCTAG101000>90>905050酶切位点保护碱基表7酶寡核苷酸序列切割率%2hr20hrSphIGGCATGCCCATGCATGCATGACATGCATGCATGT001002550StuIAAGGCCTTGAAGGCCTTCAAAAGGCCTTTT>90>90>90