高迁移率族蛋白n2对人舌癌裸鼠移植瘤抑瘤作用探究

《高迁移率族蛋白n2对人舌癌裸鼠移植瘤抑瘤作用探究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、高迁移率族蛋白N2对人舌癌裸鼠移植瘤抑瘤作用探究　　[摘要]目的研究高迁移率族蛋白N2（HMGN2）对人舌癌裸鼠移植瘤生长的抑瘤作用。方法裸鼠腋下注射接种舌鳞癌细胞Tca8113以建立裸鼠移植瘤模型，接种7d成瘤后，设立阴性对照组、HMGN2蛋白组和阳性对照组，在瘤体周边分别注射含washingbufferⅡ、HMGN2蛋白和顺铂的细胞培养基。观察各组裸鼠肿瘤的生长情况，经过4次给药后处死裸鼠，取出瘤块，称其质量计算抑瘤率，并行苏木精-伊红（HE）染色观察形态学变化。结果成功建立舌癌裸鼠移植瘤模型。HMGN2蛋白组和阳性对照组肿

2、瘤体积明显小于阴性对照组。裸鼠体重在整个实验过程中并没有明显的变化。阴性对照组、HMGN2蛋白组、阳性对照组的肿瘤质量平均值分别为（0.38±0.19）、（0.21±0.15）、（0.23±0.16）g，3组间的差异无统计学意义。HMGN2蛋白组和阳性对照组的抑瘤率分别为45.71%和39.44%。肿瘤经肉眼观察可见阴性对照组瘤块较大，HE染色可见HMGN2蛋白组和阳性对照组细胞坏死明显。结论HMGN2蛋白可以明显抑制人舌癌裸鼠移植瘤的生长。[关键词]高迁移率族蛋白N2；移植瘤；Tca8113细胞10[中图分类号]R730.2[

3、文献标志码]A[doi]10.7518/hxkq.2014.01.002InhibitoryeffectsofhighmobilitygroupchromosomalproteinN2onhumantonguecarcinomatransplantedinnudemiceLiuXiqian，DongXiaoqian，ZhangYonghong，ZhangPing，LiXiaoyu，ChenSixiu，FengYun.（StateKeyLaboratoryofOralDiseases，WestChinaHospitalofStom

4、atology，SichuanUniversity，Chengdu610041，China）[Abstract]ObjectiveToevaluatetheinhibitoryeffectofhighmobilitygroupchromosomalproteinN2（HMGN2）onhumantonguecarcinomatumorinnudemice.MethodsAtransplantationtumormodelinnudemicewasconstructedbyinjectingTca8113cells.Afterawe

5、ek，negativecontrolgroups，masculinecontrolgroups，andHMGN2groupswereesta-blished.CellcultureofthethreegroupswereseparatelyinjectedwithwashingbufferⅡ，cis-dichlorodiamineplatinum（DDP），andHMGN2protein.Thetumorsweremovedafterfourtreatments，andthenanalyzedbyhematoxylin-eosi

6、n（HE）staining.ResultsAtransplantedtumormodelwas10establishedsuccessfully.ThevolumesofHMGN2groupsandmasculinecontrolgroupsweresmallerthanthoseofthenegativegroups.Mouseweightdidnotdifferamongthethreegroups.Averagetumorweightofthenegativegroupwas（0.38±0.19）g，thatoftheHM

7、GN2groupwas（0.21±0.15）g，andthatoftheDDPgroupwas（0.23±0.16）g.Thesefactorsindicatednostatisticallysignificantdifferenceamongthethreegroups.ThetumorinhibitoryrateofHMGN2groupwas45.71%，andthatofthepositivegroupwas39.44%.Basedonevaluationbynakedeye，thetumorinthenegativegr

8、oupwaslargerthanthatinothergroups.Inaddition，cellnecrosiswasobservedduringHEstaining.ConclusionHMGN2couldsignificantlyinhibitgrowth