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1、BiochemicalSystematicsandEcology50(2013)147–153ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoTheeffectivenessofISSRprofilingforstudyinggeneticdiversityofAspergillusflavusfrompeanut-cropp
2、edsoilsinChinaa,baabChu-ShuZhang,Fu-GuoXing,JonathanNimalSelvaraj,Qing-LiYang,aaa,*LuZhou,Yue-JuZhao,YangLiuaDeptofFoodSafety&QualityControl,InstituteofAgro-ProductsProcessingScienceandTechnology,ChineseAcademyofAgriculturalSciences(CAAS),No.2YuanMingYuanWestRoad,Haid
3、ianDistrict,100193Beijing,PRChinabShandongPeanutResearchInstitute,Qingdao266100,PRChinaarticleinfoabstractArticlehistory:Weusedmarkersbasedoninter-simplesequencerepeats(ISSR)toexaminethegeneticReceived21November2012diversityofAspergillusflavusfrompeanut-croppedsoilsinC
4、hina.Ofthe100primers,22Accepted28March2013primersproducedclearandreproducibleISSRbands,andthedi-nucleotideaccountedforAvailableonline73%ofthoseprimers.ThesizeofDNAfragmentsrangedfrom100to2000bp.TheprimerUBC834producedthelargestnumberofpolymorphicbands(10),followedbyUB
5、C809,Keywords:UBC817,UBC895,andUBC899,whichallamplified7polymorphicbands.UsingthefiveInter-simplesequencerepeats(ISSR)primers,thetestedstrainswereclearlyseparatedbasedongeneticsimilaritycoefficientsAspergillusflavus(GSC).TherangeofGSCwasfrom0.59to0.90.Inunweightedpair-gro
6、upmethodwithPrimerscreeningGeneticdiversityarithmeticaverages(UPGMA)analysis,theA.flavussamplesgroupedinfiveclusters.ThestudyshowedthattheISSRtechnologyisaneffectivemolecularapproachforstudyingdiversityofA.flavusfrompeanut-croppedsoilsinChina.Ó2013ElsevierLtd.Allrightsre
7、served.1.IntroductionAspergillusflavusisanascomycetethatinfectsmanyeconomicallyimportantcrops,includingcorn,cotton,peanuts,andmanytreenuts(Horn,2003).ManystrainsofA.flavuscanproducecarcinogenicmycotoxins,whichcanseriouslyaffecthumanhealth.Understandinghowthecapacityform
8、ycotoxinproductionrelatestostraindiversitywillbecriticaltoeffectivemanagementofthishazard.Recentlydevelopedmoleculartechniqu
