欢迎来到天天文库
浏览记录
ID:7862787
大小:70.50 KB
页数:6页
时间:2018-03-01
《基因工程试卷.doc》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、2006~2007学年第二学期(A卷)一、名词解释(每题4分,5小题,共20分)1.Blotting:Blottingistheprocessofidentityingspecificbandsofintrerestamongstallthebandsboundtothenitrocellulose.Donewithspecificprobesorantibodies.2.Probe:ProbeisaspecificDNAorRNAfragmentwhichcanbindwiththesampleDNAorRNAfordection.3.Cosmid:Plasmidshavebee
2、nconstructedwhichcontainafragmentofλDNAincludingthecossite.Theseplasmidshavebeentermedcosmidsandcanbeusedasgene-cloningvectorsinconjunctionwiththeinvitropackingsystem.4.SDSequence:ThespecialDNAsequencewhichbindsto3`endof16SrRNAandinitiatessynthesisofprotein.5.Inclusionbody:“Inclusionbodies"typ
3、icallyreferstoaggregatesofproteininbacterialcytoplasm.Inclusionbodiesareclassicallythoughttobecomprisedofmisfoldedprotein二、填空题(每题2分,5小题,共10分):1.RACE:cDNA末端快速扩增2.ori:复制原点(复制起始位点)3.MCS:多克隆位点4.PCR:聚合酶链式反应5.cccDNA:共价闭合环状DNA三、单选题(每题1分,25小题,共25分)1.Prokaryotesandeukaryotesuseseveralmethodstoregulateg
4、eneexpression,butthemostcommonmethodis() A.translationalcontrolB.transcriptionalcontrol C.posttranscriptionalcontrolD.controlofmRNApassagefromthenucleus2.Whateffectwouldtheadditionoflactosehaveonarepressedlacoperon?() A.TheoperatorsiteontheoperonwouldmoveB.Itwouldreinforcetherepressionoftha
5、tgene C.Thelacoperonwouldbetranscribed D.Itwouldhavenoeffectwhatsoever3.CuttingcertaingenesoutofmoleculesofDNArequirestheuseofspecial()A.degradingnucleasesB.restrictionendonucleasesC.eukaryoticenzymesD.viralenzymes4.ADNAlibraryis() A.ageneralcollectionofallgenessequencedthusfar.B.acollect
6、ionofDNAfragmentsthatmakeuptheentiregenomeofaparticularorganism.C.aDNAfragmentinsertedintoavector.D.allDNAfragmentsidentifiedwithaprobe.5.Aprobeisusedinwhichstageofgeneticengineering?() AcleavingDNAB.recombiningDNAC.cloningD.screening6.Theenzymeusedinthepolymerasechainreactionis()A.restrict
7、ionendonucleaseB.reversetranscriptase C.DNApolymeraseD.RNApolymerase7.下列对DNA聚合酶的描述哪一种是错误的()A.具有5`-3`外切活性B.具有5`-3`聚合活性C.具有3`-5`外切活性D.具有3`-5`聚合活性8.大肠杆菌mRNA上起始密码子上游的SD序列可与某种RNA的3`端配对,然后启动多肽链合成,这种RNA是()A.tRNAB.SnRNAC.16sRNAD.23srRNA9.反转录酶
此文档下载收益归作者所有