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1、中国科技论文在线http://www.paper.edu.cn富氢盐水对东莨菪碱诱导大鼠干眼模型眼#表保护作用**楚莹莹，华宁，茹玉莎，赵少贞5（天津医科大学眼科医院，天津医科大学眼视光学院，天津医科大学眼科研究所，天津，300384）摘要：目的探讨富氢盐水(HRS)对东莨菪碱诱导大鼠干眼模型眼表保护作用。方法6周龄Wistar大鼠30只(60眼)随机分为正常组、干眼组、HRS点眼组、生理盐水(NS)点眼组、10HRS腹腔注射组和NS腹腔注射组。皮下注射东莨菪碱制备干眼模型。各组行泪液分泌试验(SIt)、泪膜破裂时间(BUT
2、)、角膜上皮荧光素钠染色评分；行HE染色、过碘酸-雪夫(PAS)染色及免疫组化染色。采用方差分析及LSD检验。结果比较各组SIt、BUT及荧光素钠染色评分差异具统计学意义(P<0.05)。HRS点眼组和HRS腹腔注射组均较干眼组改善；且二者差异无统计学意义。HE染色示HRS点眼组和HRS腹腔注射组角结膜上皮水肿减轻；PAS15染色示杯状细胞形态改善；免疫组化染色示鳞状上皮化生减轻。结论HRS点眼和HRS腹腔注射均缓解大鼠干眼症状，改善角结膜组织病理损伤，为干眼治疗带来新希望。关键词：干眼；富氢盐水；东莨菪碱；大鼠中图分类号：
3、R7720Theprotectionofhydrogen-richsalineonratdryeyemodelinducedbyscopolaminehydrobromide1122CHUYingying,HUANing,RUYusha,ZHAOShaozhen(1.TianjinMedicalUniversityEyeHospital，SchoolofOptometryandOphthalmologyofTianjinMedicalUniversity，TianjinMedicalUniversityEyeInstitut
4、e，Tianjin300384，China;252.TianjinMedicalUniversityEyeHospital，SchoolofOptometryandOphthalmologyofTianjinMedicalUniversity，TianjinMedicalUniversityEyeInstitute，Tianjin300384)Abstract:ObjectiveToevaluatetheeffectofhydrogen-richsaline(HRS)ondryeyeratinducedbysubcutane
5、ousinjectionofscopolaminehydrobromide.MethodsExperimentresearch.ThirtyfemaleWistarratsaboutsixweeksoldwererandomlydividedintonormalgroup,dryeyegroup,HRS30eyedropsgroup,normalsalineeyedropsgroup(NS),HRSintraperitonealinjectiongroupandNSintraperitonealinjectiongroup,
6、with5ratsineachgroup.Thedryeyeratmodelwasinducedbysubcutaneousinjectionofscopolaminehydrobromideinthelatterfivegroups.Theclinicalsignsofdryeyesuchastearvolume(SIt),tearbreak-uptime(BUT)andcornealepithelialfluoresceinstainingscoreswereevaluatedonday7,14,21and28.Onth
7、e28thday，teneyesineachgroupwereenucleatedand35processedforparaffinsectionsforHEandPASandImmunohistochemistrystaining.ANOVAwasusedtotestthecomparisonsoftheclinicalsigns,andLSDwasusedtotestmultiplecomparisons．ResultsFordryeyegroup,fromthe7thdayuntilthe28thday,theSIt,
8、BUTandfluoresceinstainingscoringofdryeyegroupweresignificantlydifferentfromthenormalcontrol(P<0.05).ForHRSeyedropsgroup,TheBUTwasshorterandfluore
