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《亚致死浓度甲维盐胁迫对甜菜夜蛾幼虫解毒酶系活力及其相关基因表达量的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、棉花学报CottonScience2015袁27渊2冤院149―158亚致死浓度甲维盐胁迫对甜菜夜蛾幼虫解毒酶系活力及其相关基因表达量的影响1,211*111戴瀚洋袁孙洋袁柏立新袁赵静袁肖留斌袁谭永安(1.江苏省农业科学院植物保护研究所袁南京210014曰2.南京农业大学植物保护学院袁南京210095)摘要院为明晰几种重要解毒酶在甜菜夜蛾针对甲维盐抗性形成过程中的可能作用袁本文研究分析了甲维盐处理后甜菜夜蛾幼虫体内主要解毒酶活力及其对应基因表达量的变化趋势遥结果表明袁在甲维盐(剂量为院5尧20尧50)胁迫72h后袁甜菜夜蛾体内多功能氧化酶渊MFO冤比活力及450表达量均较对照
2、极显著上升(＜0.01)袁且二者均随着处理浓度的增大而先上升后下降遥同时袁谷胱甘肽S轧转移酶渊GST冤的比活力也呈现出相同的变化趋势遥而与1在胁迫后的表达趋势与GST比活力的并不一致袁表达量随着处理浓度的上升而极显著升高(＜0.01)袁而1则随着处理浓度的上升而下降遥在3个亚致死浓度甲维盐处理下袁酯酶渊EST冤比活力均被显著抑制(＜0.05)袁同样的趋势也反映在表达量的显著变化上(＜0.05)遥此外袁MFO尧EST的比活力变化趋势与其对应基因450尧表达谱之间存在极显著相关性(＜0.01)曰而GST活力只与表达量具有极显著相关性(＜0.01)袁与1表达量之间则不存在显著相关性
3、遥因此袁MFO尧GST及其相关基因450尧尧1有可能参与到甜菜夜蛾对甲维盐抗药性的演化过程中遥关键词院甜菜夜蛾曰甲维盐曰酶活曰表达量分析曰杀虫剂胁迫中图分类号院S435.622文献标志码院A文章编号院1002-7807(2015)02-0149-10院10.11963/issn.1002-7807.2015020081,211*111DaiHanyang,SunYang,BaiLixin,ZhaoJing,XiaoLiubin,TanYong'an(1.210014,;2.210095,)Toclarifytheroleofseveralimportantenzymaticd
4、etoxificationstepsintheformationofresistanceagainstemamectinbenzoatein,thedetoxifyingenzymeactivitiesandtheexpressionofrelatedgeneswereanalyzedinlarvaetreatedwithemamectinbenzoate.When3rdinstarlarvaeofweretreatedwithemamectinbenzoateatinductiondosesofLC5,LC20andLC50for72h,themixed-functiono
5、xidase(MFO)activityandtheexpressionof450genesweresignificantlyincreased(＜0.01).Thechangesinactivityorexpressionallincreasedatfirstandthendecreasedfollowingthein-creasingdosesofemamectinbenzoate.Thechangeinglutathione-S-transferase(GST)activityofwassimilartothatforMFO,whiletheexpressiontrend
6、sforand1weredifferentfromtheGSTactivity.Comparedwiththecontrol,theexpressionofsignificantlyincreasedintreatedwithincreasinginductiondosesofemamectinbenzoate(＜0.01),whileexpressionof1decreased.Theesteraseactivitiesoftreatedwiththreeinductiondosesofe-mamectinbenzoateallsignificantlydecreased(
7、＜0.05),asdidtheexpressionof(＜0.05).Correlationanalysisindicat-edthattherewassignificantcorrelationbetweenMFO,esteraseactivitiesandtheexpressionofthecorrespondinggenes450andE,respectively(＜0.01).TherewassignificantcorrelationbetweenGSTactivitiesandexpress
