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1、中国水稻科学(ChinJRiceSci),2017,31(4):355－363http://www.ricesci.cnDOI:10.16819/j.1001-7216.2017.6165355水稻白条纹突变体st13的表型分析及基因定位1,31,3111111,2,*孙立亭林添资王云龙牛梅胡婷婷刘世家王益华万建民1(南京农业大学作物遗传与种质创新国家重点实验室/农业部长江中下游粳稻生物学与遗传育种重点实验室/长江流域杂交水稻协同创新中心/江苏省23现代作物生产中心，南京210095；中国农业科学院
2、作物科学研究所，北京100081；江苏丘陵地区镇江农业科学研究所，江苏句容212400；*通讯联系人，E-mail:wanjm@njau.edu.cn,wanjianmin@caas.cn)PhenotypicAnalysisandGeneMappingofaWhiteStripeMutantst13inRice1,31,311111SUNLiting,LINTianzi,WANGYunlong,NIUMei,HUTingting,LIUShijia,WANGYihua,1,2,*WANJianmi
3、n1(StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUniversity/KeyLaboratoryofBiology,GeneticsandBreedingofjaponicaRiceinMid-lowerYangtzeRiver,MinistryofAgriculture/TheYangtzeRiverValleyHybridRiceCollaborationInnovationCenter/
4、Jiangsu2CollaborationInnovationCenterforModernCropProduction,Nanjing210095,China;InstituteofCropScience,ChineseAcademyofAgriculturalSciences,3*Beijing100081,China;ZhenjiangAgriculturalResearchInstitute,Jurong212400,China;Correspondingauthor,E-mail:wanj
5、m@njau.edu.cn,wanjianmin@caas.cn)Abstract:【Objective】Riceleafcolormutationidentificationandcloningofrelatedgenesishelpfultoresearchphotosynthesisandfurtherunderstandthemechanismofchloroplastdevelopmentandpigmentsynthesispathway,providingtheoreticalbasi
6、sforthebreedingofricewithhighphotosyntheticefficiency.【Method】Asomaclonalmutant(designatedasstripeleaf13,st13)wasisolatedfromtissue-culturedprogeniesofajaponicaricevarietyDongjin.Themainagronomictraitsofwildtypeandst13weredeterminedatmaturity,andthepig
7、mentcontentsandultrastructureofchloroplastwereobservedattheseedlingstage.Dongjinandst13werereciprocallycrossedtoobservethephenotypeofF1andgeneticanalysiswascarriedout.AnF2populationandF2:3populationderivedfromthecrossst13×Nanjing11(anindicaricevariety)
8、wereusedforgenemappingofST13.QuantitativeRT-PCRwascarriedouttoanalyzetherelativeexpressionofgenesassociatedwithchloroplastdevelopmentandchlorophyllbiogenesisinthewild-typeDongjinandst13mutant.【Result】Comparedtothewildtype,theplantheight
