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1、中国水稻科学(ChinJRiceSci),２０１４,２８(５):４６５－４７２http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１４．０５．００３４６５水稻类病变突变体g３０３的鉴定和基因定位刘林１,＃张迎信１,＃李枝１,２刘群恩１余宁１孙滨１杨正福１周全１程式华１曹立勇１,∗(１中国水稻研究所/国家水稻改良中心/浙江省超级稻研究重点实验室,杭州３１０００６;２杭州师范大学生命与环境科学学院,杭州３１００３６;＃共同第一作者;∗通讯联系人,EＧma
2、il:caolycgf＠mail．hz．zj．cn)CharacterizationandGeneMappingofaLesionMimicMutantg３０３inRiceLIULin１,＃,ZHANGYingＧxin１,＃,LIZhi１,２,LIUQunＧen１,YUNing１,SUNBin１,YANGZhengＧfu１,ZHOUQuan１,CHENGShiＧhua１,CAOLiＧyong１,∗(１ZhejiangKeyLaboratoryofSuperRice/NationalCenterforR
3、iceImprovement/ChinaNationalRiceResearchInstitute,Hangzhou３１０００６,China;２CollegeofLifeandEnvironmentalSciences,HangzhouNormalUniversity,Hangzhou３１００３６,China;＃Theseauthorscontributedequallytothispaper;∗Correspondingauthor,EＧmail:caolycgf＠mail．hz．zjc．n)LIU
4、Lin,ZHANGYingxin,LIZhi,etal．Characterizationandgenemappingofalesionmimicmutantg３０３inrice．ChinJRiceSci,２０１４,２８(５):４６５Ｇ４７２．Abstract:Thelesionmimicmutantg３０３andg３４２wereisolatedbytreatingtheseedsofindicavarieties９３１１andR８０１５withγＧrayradiation,respectively．T
5、heyellowspotsinitiallyappearong３０３leavesat４Ｇleafstage．Withthegrowthanddevelopmentoftheplant,thelesionsalmostoccupiedthewholeplanttillthematuritystage．Comparedwiththewildtype,theplantheight,seedsettingrateand１０００Ｇgrainweightofg３０３significantlydropped,the
6、chlorophyllandphotosyntheticrateobviouslydecreased．Geneticanalysisshowedthatthemutantphenotypewascontrolledbyasinglerecessivegene．UsingF２mappingpopulationofg３０３/Nipponbare,theg３０３mutantgenewasmappedbetweenmarkerInD１０andInD１２onricechromosome１２,withgeneti
7、cdistancesof０．１９cMand０．７６cM,respectively．SequenceanalysisrevealedthatthemutatedgenewasallelictoSL,g３０３andg３４２mutatedgenehadasinglenucleotidedeletion(T５７２andG１２０６,respectively),leadingtoaprematureterminationcodon．RealＧtimePCRanalysisshowedthattheexpressi
8、onlevelofdefenseＧrelatedgenesupregulatedsignificantly,theseresultsdemonstratedthatthemutationmayactivatethedefenseresponse．Keywords:rice;lesionmimicmutant;genemapping;defenseresponse刘林,张迎信,李枝,等．水稻类病变突变体g３０３的鉴定和基因定位．中国水稻科学,２０１４,２８
