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1、中国水稻科学(ChinJRiceSci),２０１６,３０(５):４７９－４８６http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．６０３７４７９水稻矮秆多分蘖突变体mz３的遗传分析和基因定位苏晓妹１方宇星１刘钰龙１刘峰１张所兵２张云辉２,∗鲍依群１,∗(１南京农业大学生命科学学院,南京２１００９５;２江苏省农业科学院粮食作物研究所/江苏省农业种质资源保护与利用平台,南京２１００１４;∗通讯联系人,EＧmail:baoyiqun＠njau．edu．cn,zyhrice＠１６３．com)GeneticAnalysisandGeneMappi
2、ngofaDwarfandHighＧtilleringMutantmz３inRice(OryzasativaL．)SUXiaoＧmei１,FANGYuＧxing１,LIUYuＧlong１,LIUFeng１,ZHANGSuoＧbing２,ZHANGYunＧhui２,∗,BAOYiＧqun１,∗(１CollegeofLifeSciences,NanjingAgriculturalUniversity,Nanjing２１００９５,China;２JiangsuProvincialPlatformforConservationandUtilizationofAgriculturalGermpla
3、sm/InstituteofFoodCrops,JiangsuAcademyofAgriculturalSciences,Nanjing２１００１４,China;∗Correspondingauthor,EＧmail:baoyiqun＠njaue．duc．n,zyhrice＠１６３c．om)SUXiaomei,FANGYuxing,LIUYulong,etal．GeneticanalysisandgenemappingofadwarfandhighＧtilleringmutantmz３inrice(OryzasativaL．)．ChinJRiceSci,２０１６,３０(５):４７９Ｇ４
4、８６．Abstract:AdwarfandhighＧtilleringmutant,temporarilytermedasmz３wasobtainedfromZhonghua１１,ajaponicaricevarietybyEMSmutagenesis．Geneticanalysisindicatedthatthemutantphenotypewascontrolledbyarecessivegene．AnF２populationwasdevelopedbycrossingmz３withNanjing１１,anindicaricevarietyformappingofthemutant
5、gene,whichwasultimatelyrestrictedwithinaphysicaldistanceofabout７４７kbbetweentwoSSRmarkersRM１９３５３andRM５１０onthelongarmofricechromosome６．GenepredictionrevealedthatD３,ageneconferringriceplantheightandtillernumberwaslocatedinthisregion．SequenceanalysisidentifiedasingleGtoAnuclearacidsubstitutionatthep
6、osition６３６fromtheATGstartcodon,formingaprematuretranslationalterminationcodon．Sequencingof１０randomlyselectedrecessiveplantsfrommz３/Nanjing１１mappingpopulationconfirmedthesamemutationsitecarriedbythe１０individuals．SubcelluarlocationindicatedthatD３proteinofthemutantwaslocatedinthenucleiaswildtype．Bi
7、FCanalysisshowedthatD３proteinofthemutantcouldnotinteractwithD１４proteinforlackingofessentialaminoacidsthatbindwithD１４,blockingsignaltransductionofstrigolactones．Sowespeculatedthatthemutantphenotypeofmz３wasprobablycausedbymuta
