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1、中国水稻科学(ChinJRiceSci),２０１６,３０(５):５４１－５５１http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１９８５４１稻曲病菌TＧDNA插入突变体B２５１０的插入位点分析丁慧１,２俞咪娜２王亚会２于俊杰２尹小乐２薄惠文１,２黄星１刘永锋２,∗(１南京农业大学生命科学学院,南京２１００９５;２江苏省农业科学院植物保护研究所,南京２１００１４;∗通讯联系人,EＧmail:liuyf＠jaas．ac．cn)MolecularCharacterizationofUstilaginoideavirensTＧDNAInse
2、rtionMutantB２５１０DINGHui１,２,YUMiＧna２,WANGYaＧhui２,YUJunＧjie２,YINXiaoＧle２,BOHuiＧwen１,２,HUANGXing１,LIUYongＧfeng２,∗(１CollegeofLifeScience,NanjingAgriculturalUniversity,Nanjing２１００９５,China;２InstituteofPlantProtection,JiangsuAcademyofAgriculturalSciences,Nanjing２１００１４,China;∗Correspondingauthor,EＧmail:
3、liuyf＠jaas．acc．n)DINGHui,YUMina,YUJunjie,etal．MolecularcharacterizationofUstilaginoideavirensTＧDNAinsertionmutantB２５１０．ChinJRiceSci,２０１６,３０(５):５４１Ｇ５５１．Abstract:ToisolatethegeneconcernedwithmolecularpathogenicprocessofUstilaginoideavirens(U．virens),TＧDNAintegrationflankingsequenceandthemutantgene
4、sofamutantstrainB２５１０wereanalyzed．ComparedwiththewildＧtypeU．virensstrainP１,thepathogenicityofthemutantstrainB２５１０inthefieldwassignificantlydecreased．ThegrowthrateofB２５１０onMMmediumwasslowerthanthatofP１,butwasnotsignificantlydifferentonPSAandTB３mediumwhichwerenutritionallyendowed．ThemutantstrainB２
5、５１０didnotproduceconidiophoresinPSbrothmedium．GenomicSouthernboltanalysisconfirmedthatthereweredoubleTＧDNAeventsinsertedingenomeofmutantstrainB２５１０．TheflankingU．virenssequencesofTＧDNAobtainedbyTAILＧPCRwereadjacentinthewildtypeandwithnosequenceslost,andonlyafewbasesofTＧDNAwerechanged．TheTＧDNAinser
6、tionsitewasinthepromoterregionofUV８b_１４１２anddownstream３′regionofUV８b_１３８６,respectively．RTＧPCRanalysisconfirmedthattheexpressionofbothoftwogenesinB２５１０weresignificantlydecreased．ThegeneswhichwereaffectedbyTＧDNAinsertionmaybeassociatedwithpathogenicityandparticipateintheregulationofpathogenicproce
7、ssofU．virensinrice．Keywords:Ustilaginoideavirens;TＧDNAinsertionmutant;ATMTtransformation;pathogenicity;flankingsequence丁慧,俞咪娜,王亚会,等．稻曲病菌TＧDNA插入突变体B２５１０的插入位点分析．中国水稻科学,２０１６,３０(５):５４１Ｇ５５１．摘要:以稻曲病菌TＧDNA插入突变体库中致病力减弱突变菌株B２５１０为材料,通
