聚合水稻温敏核不育基因和反温敏核不育基因创制永久核不育系

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1、中国水稻科学(ChinJRiceSci),2017,31(4):371－378http://www.ricesci.cnDOI:10.16819/j.1001-7216.2017.7017371聚合水稻温敏核不育基因和反温敏核不育基因创制永久核不育系*江建华倪金龙吴爽王德正*(安徽省农业科学院水稻研究所/国家水稻改良中心合肥分中心/安徽省水稻遗传育种重点实验室，合肥230031；通讯联系人，E-mail：wdzhengqin@163.com)DevelopmentofPermanentGenicMaleSterileLinebyPyramidingThermo-sensi

2、tiveGenicMaleSterileGenesandReverseTemperatureInducedGenicMaleSterileGenesinRice(OryzasativaL.)*JIANGJianhua,NIJinlong,WUShuang,WANGDezheng(InstituteofRiceResearch,AnhuiAcademyofAgriculturalSciences/HefeiSub-centerofChinaNationalRiceImprovementCenter/AnhuiKeyLaboratoryof*RiceGeneticsandBr

3、eeding,Hefei230031,China;Correspondingauthor,E-mail:wdzhengqin@163.com)Abstract：【Objective】Thesecurityproblemofseedproductionhasseriouslyhamperedthesteadilyandsustainabledevelopmentoftwo-linehybridrice.【Method】AiziS(thermos-sensitivegenicmalesterile,TSGMS)andAiyans(reversetemperaturesensi

4、tivegenicmalesterileline,RTSGMS)werescreenedastheoriginalparentstodeveloppermanentgenicsterileline(PGSL)accordingtotheF1fertilityofcrossesamong16(light-)TSGMSand4RTSGMS.ThefertilitygeneswereintroducedintothenearisogeniclinesTianfengSandTianfengsbybackcrossingAiziSandAiyanswiththebridgepar

5、entTianfengB,respectively.Then,TianfengSs,thePGSL,wasobservedfromthecrossbetweenTianfengSandTianfengs.Thefertility,restoration,competitiveadvantageofyield-relatedtraitsandreproductivecharacteristicsofTianfengSswereinvestigated.【Result】TianfengSswascompletelysterileundernaturallongdaywithh

6、ightemperatureandshortdaywithlowtemperature.AndnoobviousdifferencewasnotedamongtheF1crossesfromTianfengSsanditsthreeparentsandfiverestorersinspikeletfertilitypercentage.【Conclusion】TheresultsshowedthatthenewlydevelopedPGSLmightsolvethesecurityproblemofseedproductionintwo-linehybridricefun

7、damentally.Keywords：rice;thermo-sensitivegenicmale;reversethermo-sensitivegenicmale;permanentgenicsterileline摘要：【目的】制种安全是影响两系杂交稻持续健康发展的关键问题。本研究旨在探索解决两系杂交水稻的制种安全隐患途径。【方法】以16个（光）温敏核不育系与4个反温敏核不育系配组并对F1育性进行观察，选择矮紫S（温敏核不育系）和矮雁s（反温敏核不育系）为构建永久核不育系的供源亲本。通过回交将矮紫S和矮雁s的育性基因导入到