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1、OpenJournalofApoptosis,2013,2,25-30http://dx.doi.org/10.4236/ojapo.2013.23004PublishedOnlineJuly2013(http://www.scirp.org/journal/ojapo)ComparisonofTwoRenalCellLines(NRK-52EandLLC-PK1)asLateStageApoptosisModelsAlexandraH.Heussner,DanielR.Dietrich*HumanandEnviro
2、nmentalToxicology,UniversityofKonstanz,Konstanz,Germany*Email:daniel.dietrich@uni-konstanz.deReceivedMarch12,2013;revisedApril12,2013;acceptedApril22,2013Copyright©2013AlexandraH.Heussner,DanielR.Dietrich.ThisisanopenaccessarticledistributedundertheCreativeComm
3、onsAttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.ABSTRACTRenalcelllinesarefrequentlyusedmodelsintoxicology.Theaimoftheexperimentsdescribedherewastoinvestigatethesuitabilityoftwoofth
4、oserenalcelllines,namelyNRK-52EandLLC-PK1,asmodelsformidtolatestageapop-tosisunderstandardcellcultureconditions;thelattermeansthattestingwasperformedinthepresenceofserumintheculturemedia.Sevenknowninducersofapoptosisalreadypositivelytestedbyotherinvestigatorswe
5、rechosenastestsubstancesusingchromatincondensation(Hoechststaining)asendpoint.Thesesubstanceswerecadmiumchloride(CdCl2),dithiothreitol(DTT),sodiumchloride(NaCl),mercuricchloride(HgCl2),tributyltinoxide(TBT-O),tributyltinchloride(TBT-Cl)andstaurosporine.Fromthes
6、e,onlyTBT-O,TBT-Clandstaurosporineinducedmorphologicalfea-turestypicalofapoptosisinLLC-PK1cells.MorphologicallydiscernedapoptosiswasconfirmedbyDNAfragmenta-tion(DNAladderingassay)analysis.LLC-PK1cells,butnotNRK-52Ecells,wereshowntobesuitablemodelsofmidtolatesta
7、geapoptosisundertheconditionsemployed.TBT-O,TBT-Clandstaurosporinewereshowntobesuitablepositivecontrolsforapoptosisinrenalcellsinvitro.Keywords:RenalCellLines;Hoechst33342;DNALaddering1.Introductioncondensedchromatinofapoptoticcellsmorebrightlythanthechromatino
8、fnormalcells.Condensedchromatinwithadistinctfragmentsize,roughly180-200basepairsandmultiplesthereof,istheresultofaspecificDNAfragmentationviacleavagebyendogenousen-donucleas
