缺氧复氧诱导大鼠肾小管上皮细胞内质网应激反应.doc

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1、内质网应激在大鼠肾小管上皮细胞缺氧复氧损伤炎症反应中的作用童玉娜，杨聚荣，景宇，张建国，李开龙，何娅妮(400042重庆，第三军医大学大坪医院野战外科研究所肾内科)[摘要]目的:观察大鼠肾小管上皮细胞（NRK-52E细胞）缺氧复氧损伤时内质网应激蛋白和炎症细胞因子表达的改变及其意义,以探讨缺氧复氧诱导大鼠肾小管上皮细胞内质网应激与炎症反应的关系。方法:NRK-52E细胞用含5%胎牛血清的DMEM液培养于37℃、5%CO2恒温培养箱。将细胞分为正常对照组和缺氧/复氧损伤（hypoxiareoxygenation，H/R）组。采用

2、全自动生化分析仪检测细胞培养液上清LDH活性，Westernblot方法检测GRP78、CHOP蛋白表达，ELISA法检测细胞上清IL-β水平。结果:①与对照组细胞培养液上清LDH[（22.07±1.23）U/L]比较，H/R组[（45.93±6.42）U/L]显著增加(PP为大写斜体字母，请全文修改<0.05)。②与对照组肾小管上皮细胞GRP78表达(0.80±0.04)比较,H/R组(1.24±0.04)明显增加(P<0.05)；与对照组肾小管上皮细胞CHOP表达(0.81±0.05)比较,H/R组(1.19±0.05)明

3、显增加(P<0.05)。③与对照组IL-1β水平（6.55±2.03）比较，H/R组（14.93±2.26）显著增加(P<0.01)。结论:缺氧复氧可以诱导大鼠肾小管上皮细胞内质网应激与炎症反应。内质网应激上调CHOP表达可能在肾小管上皮细胞缺氧复氧损伤炎症反应中具有重要作用。[关键词]缺氧复氧；内质网应激；肾小管上皮细胞；CHOP；炎症Roleofendoplasmicreticulumstressinhypoxia/reoxygenationinducedinflammationinratrenaltubularepith

4、elialcellsTongYuna,YangJurong,JingYu，ZhangJianguo，LiKailong，HEYani(DepartmentofNephrology,InstituteofSurgeryResearch,DapingHospital,ThirdMilitaryMedicalUniversity,Chongqing400042,China)[Abstract]ObjectiveToinvestigatetheeffectofhypoxia/reoxygenationonendoplasmicreti

5、culumstress（ERS）andinflammatoryresponseinratrenaltubularepithelialcells（NRK-52Ecells）.MethodsNRK-52Ecellsin10%FBSDMEMculturemediumweredividedinto2groups,controlgroupandhypoxia/reoxygenation(H/R)group.CellsinH/Rgroupwereexposedtohypoxiafor4hoursandsubsequentlyreoxyge

6、nationfor12hours.LDHinculturesupernatantwasdetectedbyautomaticbiochemistryanalyzer.WesternblotwasappliedtodetecttheexpressionofGRP78andCHOP.IL-1βinculturemediumwasdetectedbyELISA.ResultsCellviabilitywasdecreasedobviouslyafterhypoxia/reoxygenation(P<0.05).Comparedwit

7、huntreatedcells,theexpressionofGRP78andCHOPproteinofthecellshadincreasedsignificantlyinthehypoxia/reoxygenationcells(P<0.05).TheexpressionofIL-1βincreaseddistinctlyinthehypoxia/reoxygenationcells(P<0.01).ConclusionHypoxia/reperfusioncaninduceendoplasmicreticulumstre

8、ssandinflammatoryresponseinratrenaltubularepithelialcells.Endoplasmicreticulumstressmaybeessentialintheinflammationofrenaltubularepithelia