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1、鸢尾黄素对肝细胞癌HepG2细胞的促进凋亡作用鸢尾黄素对肝细胞癌HepG2细胞的促进凋亡作用Pro-apoptoticeffectsoftectorigeninonhumanhepatocellularcarcinomaHepG2cells#510152025303540DINGHui,SHIDahua,LIErguang,WANGYurong,JIANGChunping,WUJunhua**JiangsuKeyLaboratoryofMolecularMedicine,StateKeyLaboratoryofPharmaceuticalBiotechnolog
2、y,MedicalSchool,NanjingUniversity,NanJing210093Abstract:Tectorigenin,amainisoflavoneintheIristectorumrhizomewhichwasusedtraditionallyfortreatingliver-relateddiseases,wasshowntoinhibitthegrowthofhumanhepatomaHepG2cells,andtoinducethecellapoptosis.Intheapoptoticprocess,theintracellularROS
3、andCa2+elevationactedasanearlyeventfollowedbydepolarizationofmitochondrialmembranepotentialMMP.Andthencytochromecwasreleasedtocytosol.Moreover,thetectorigenintreatmentco-enhancedthecaspase-3,-8and-9proteolyticactivities.Inconclusion,tectorigeninsignificantlyinhibitedtheproliferationandi
4、nducedapoptosisinhumanhepatocellularcarcinomaHepG2cellsmainlyviamitochondrial-mediatedpathway.Andthedeathreceptor-dependentpathwaymightplayacertainroleintheapoptoticprocess.TheseobservationsindicatedthattectorigeninistheactiveprincipleofIristectorumrhizomeappliedinthefolkmedicineforalle
5、viatingliverdisorders,andthattheisoflavoneisapromisingcandidateforhepatocellularcarcinomachemotherapeuticandchemopreventiveagent.Keywords:Tectorigenin;Anti-proliferation;Apoptosis;Mitochondrial;HepG20IntroductionHepatocellularcarcinomaHCCisthethirdmostcommoncauseofcancer-relatedmortalit
6、yworldwide,with600000deathsperyear[1].ItoftendevelopsinpatientswithchronicliverdiseasesassociatedwithhepatitisBHBVorhepatitisCHCVvirusinfections[1].Althoughsurgicaltechniqueshavebeenimprovedandseveralnon-surgicaltreatmentmodalitieshavebeendeveloped,thereisnoeffectivetherapyforsignifican
7、timprovementofextremelypoorprognosisofHCCpatients[2].Therefore,effortshavebeenmadetosearchformechanism-basedagents,suchassorafenib[3],chelidonine[4],5-allyl-7-gen-difluoromethylenechrysin[5],troglitazone[6],chagamushroomextract[7]andcurcumin[8],fortreatmentofHCC.Sorafenibisthef
