温阳活血方上调慢性马兜铃酸肾病大鼠肾组织血管生成素1 mRNA表达.doc

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1、温阳活血方上调慢性马兜铃酸肾病大鼠肾组织血管生成素1mRNA表达作者：王红婷,王云满,梁永平,刘育军,彭文【摘要】　　目的：观察温阳活血方对慢性马兜铃酸肾病大鼠肾组织血管生成素（angiopoietin,Ang）表达的影响，并探讨其保护作用机制。　　方法：将28只雄性SD大鼠随机分为正常对照组、模型组和温阳活血方组。模型组大鼠予关木通水煎液10ml/(kg·d)灌胃，温阳活血方组于模型组基础上，再予温阳活血方30g/(kg·d)灌胃。20周后，采用实时逆转录聚合酶链式反应方法检测3组大鼠肾组织中Angl和A

2、ng2mRNA的表达。　　结果：与正常对照组比较，模型组大鼠肾组织Ang1mRNA表达明显减少（P<0.01），Ang2mRNA表达明显升高（P<0.01）；与模型组大鼠比较，温阳活血方组大鼠肾组织Ang1mRNA表达升高（P<0.01），Ang2mRNA表达下降，但差异无统计学意义（P>0.05）；肾脏病理显示温阳活血方组肾损害较模型组明显减轻。　　结论：温阳活血方通过上调Ang1mRNA的表达发挥对慢性马兜铃酸肾病大鼠肾脏保护作用。【关键词】马兜铃酸;温阳活血方;血管生

3、成素类;关木通;大鼠;肾病　　Objective:ToinvestigatetheeffectsofWenyangHuoxueRecipe(WRHXR),acompoundtraditionalChineseherbalmedicineforwarmingyangandpromotingbloodflow,ontheexpressionofangiopoietinmRNAinratswithchronicaristolochicacidnephropathyinducedbyCaulisAristolochi

4、aManshuriensis(CAM)decoction,andtoexploretheprotectionmechanismofWYHXRagainstkidneydamage.　　Methods:TwentyeightmaleSDratswererandomlydividedintonormalcontrolgroup,CAMgroupandWYHXRtreatedgroup.Ratsinthenormalcontrolgroup(n=8)andCAMgroup(n=10)wereintragastr

5、icallyadministeredwithnormalsaline10ml/(kg·d)orCAMdecoction10ml/(kg·d)respectively.RatsintheWYHXRtreatedgroup(n=10)wereintragastricallyadministeredwithWYHXR30g/(kg·d)andCAMdecoction10ml/(kg·d).TheexpressionsofAnglandAng2mRNAsweredetectedbyrealtimerevers

6、etranscriptionpolymerasechainreaction(RTPCR)after20weektreatment.　　Results:Comparedwiththenormalcontrolgroup,theexpressionof6AnglmRNAwassignificantlydecreased,andtheexpressionofAng2mRNAwassignificantlyincreasedintheCAMgroup(P<0.01).ComparedwiththeCAM

7、group,theexpressionofAnglmRNAwasincreasedintheWYHXRtreatedgroup(P<0.01).TheexpressionofAng2mRNAhadnosignificantdifferencebetweentheCAMgroupandtheWYHXRtreatedgroup(P>0.05).RenalpathologyshowedthatrenaldamageinWYHXRtreatedgroupwassignificantlyreduc

8、edascomparedwiththeCAMgroup.　　Conclusion:WYHXRcanupregulatetheexpressionofAnglmRNA,whichmaybeitsactionmechanisminprotectingthekidneys.　　Keywords:aristolochicacid;WenyangHuoxueRecipe;angiopoietins;Cau