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1、槐定碱抗骨癌痛作用和其机制探究[摘要]目的：采用W256癌细胞诱导的骨癌痛模型大鼠探讨槐定碱抗癌痛药效及其作用机制。方法：采用骨髓腔注射W256癌细胞复制骨癌痛大鼠模型；造模10d后选取模型复制成功的大鼠36只随机分为模型对照组及槐定碱治疗组，另取同期10只大鼠作为正常对照组；治疗组大鼠于造模第15天开始给予槐定碱25mg•kg-1治疗10d;各组大鼠给药前、后测量机械痛阈和热痛阈，末次给药后，对大鼠患肢进行放射学和组织病理学观察，并采用免疫组化法检测大鼠患肢肿瘤组织环氧酶-2(COX-2)、血管内皮生长因子(VEGF)的表达。结果：槐定碱可明显提高骨癌痛大鼠机械痛阈和
2、热痛阈值(P3.2槐定碱对大鼠热痛阈的影响造模动物在术后第14天出现明显热痛觉超敏，热痛阈值显著下降(P[9]HuM,PeluffoG,Chenll,etal.RoleofCOX-2inepithelial-stromalcellinteractionsandprogressionofductalcarcinomainsituofthebreast[J].ProcNatlAcadSciUSA,2009，106：3372.[10]王勤，邓家刚，杨柯，等.芒果苷对慢性支气管炎大鼠炎症因子及小鼠巨噬细胞环氧化酶-2表达的影响[J].中国中药杂志，2011，36(10)：134
3、8.[11]SabinoMA,GhilardiJR，JongenJL，etal.Simultaneousreductionincancerpain，bonedestruction,andtumorgrowthbyselectiveinhibitionofcyclooxygenase-2[J].CancerRes,2002，62：7343.[10]王日雄，施烯，林学德，等.塞来昔布在MRMT-1大鼠乳腺癌骨转移性癌痛模型的作用机制探讨[J].中国疼痛医学杂志，2010，16(4)：228.[11]Tawfik0W，KramerB，ShidelerB，etal.Progno
4、sticsignificanceofCD44，platelet-derivedgrowthfactorreceptoralpha,andcyclooxygenase-2expressioninrenalcellcarcinoma[J].ArchPatholLabMed,2007,131：261.[12]BasuGD,PathangeyLB，MukherjeeP,etal.Mechanismsunderlyingthegrowthinhibitoryeffectsofthecyclo-oxygenase-2inhibitorcelecoxibinhumanbreastca
5、ncercells[J].BreastCancerRes,2005，7：4220.StudyoneffectofsophoridineagainstbonecancerpainanditsmechanismYANJi-gui*，YANGYu-qing,WANGYa-jie，KANJing(DepartmentofPharmacy,AnhuiMedicalCollege,Hefei230601，China)[Abstract]Objective:Tostudytheeffectofsophoridineagainstbonecancerpaininbonecancerpa
6、inmodelratsinducedbyW256tumorcellsanditsmechanism.Method：TheratmodelofbonecancerpainwasreproducedbyinjectingW256tumorcellsintotheratmarrowcavity.Tendaysafterthemodelestablishment,36ratswereselectedandrandomlydividedintothemodelcontrolgroupandthesophoridinetreatedgroup.Atthesametime,other
7、10ratswithsham-operationwereselectedtobethenormalcontrolgroup.Sincethe15thdayaftertheoperation,ratsinthetreatedgrouphadbeengivensophoridine(25mg•kg-1)for10days.Themechanicalwithdrawalthresholdandthethermalwithdrawallatencyofeachgroupweremeasuredbeforeandafterthetreatment.
