塞来昔布对嘌呤氨基核苷诱导的足突细胞凋亡影响的实验研究-内科学专业毕业论文

《塞来昔布对嘌呤氨基核苷诱导的足突细胞凋亡影响的实验研究-内科学专业毕业论文》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、江苏大学硕士论文Caspase．3活性检测试剂盒检澳lJCaspase．3蛋白酶活性水平。在24h，用Westernblot技术检测各组P53及COX．2水平。结果1．在33℃增殖状态下，足细胞呈鹅卵石状，在37℃分化状态下的足细胞呈分叉状。正常足细胞在33℃表达COX．2，在37℃分化14d后，用低血清培养液培养后，随着时间延长，COX．2表达逐渐增多，24h达最高，以后再逐渐减少。2．与正常对照组相比较，DEX、CELE组凋亡率无明显变化(P>0．05)，PA组凋亡率明显增高(P<0．001)，DEX+PA、CELE+PA组凋亡率

2、较PA组明显下降(P<0．05)。3．各组Caspase一3活性无明显差异(P>0．05)。4．24h时，PA组P53表达较正常对照组明显增多，用DEX、CELE干预后其表达明显降低(P<0．05)。5．正常对照组即可表达COX．2，用PA诱导24h，COX．2表达明显增加，用DEX、CELE干预后COX．2表达水平有所减少(P<0．05)。结论1．COX．2在足细胞上的表达呈时间依赖性。2．塞来昔布可以抑制PA诱导的足细胞凋亡，P53参与了PA诱导的足细胞凋亡过程。塞来昔布抑制PA诱导的足细胞凋亡可能与足细胞所表达的COX．2水平有

3、一定的相关性。关键词：塞来昔布，足细胞，细胞凋亡，环加氧酶2II江苏大学硕士论文ABSTRACTBackgroundTheglomerularvisceralepithelialcell，alsocalledaspodocyte，isahi曲lyspecializedandterminallydifferentiatedcellwhichplaysamajorroleinestablishingtheglomerularfiltrationbarrier．Podocytesthereforehavelimitedabilitytobe

4、replacediflost．Thereisagrowingbodyofliteratureshowingthereducedpodocytenumberisacriticaldeterminantunderlyingthedevelopmentofglomerulosclerosis．Decreasedpodocytenumberiscausedbydetachmentofcellsfromtheglomerularbasementmembraneand／orbyapoptosis．Theunderlyingmechanismsof

5、whichthespecificcyclooxygenase一2(cox一2)inhibitorshavebeenshowntodecreaseproteinuriaandretardprogressiveglomerularinjuryinsomeanimalmodelshavenotbeenaddressedformally．Accordingly,inthisstudyweinvestigatedthedirecteffectofcelecoxib，aspecificCOX一2inhibitors，onpodocyteapopt

6、osisinducedbypuromycinaminonucleoside(PA)andthemechanismsthatunderliethiseffectinordertofurthersearchforclinicaltherapeutictargetonchronicprogressiveglomerularlnJury．Methods1．Experimentswereperformedusingearly-passagegrowth-restricted，conditionallyimmortalizedmousepodoc

7、ytes．TheexpressionofCOX一2III江苏大学硕士论文inpodocyteswasmeasuredbyuseofWesternblotanalysis．TheeffectsofspecificCOX一2inhibitorscelecoxibonpodocyteapoptosisinducedbyPAwillbeexaminedonthebasisofpodocyteculture．2．Theconditionallyimmortalizedmousepodocyteswerestudiedanddividedinto

8、sixgroups．Control(CON)；puromycinaminonucleoside(PA)；dexamethasone(DEX)；celecoxib(CELE)；dexamethasone+puromycin