molecular weight protein size.pdf

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1、ProteinsizeMolecularweightforproteinsTheultimateindesktopproteincharacterizationdetailedspecificationsfromwww.malvern.com/proteinsMakinglightworkforproteinsAddthepowerofadvancedlightscatteringtechnologytoyourproteinlaboratoryincludingabsolutesizingforsizeexclusionchromatography.Howdoyouknowift

2、heproteiniswell-behavedinthebuffer?Whatisthetrueformoftheproteininsolution?Improveaccuracy,reliabilityandeaseofusewiththemostadvancedsystemavailable–theZetasizerNano.—Amaintenancefreesystemideallysuitedtoyourproteincharacterizationneeds—AttachtoyourSEC/GPCsystemtouseasanabsolutesizingdetectorM

3、olecularweightUsingstaticlightscattering(SLS)andtheclassicalDebyeplot,themolecularweightofglobularproteinsupto2x107Daandrandomcoiledpolymersupto5x105DacanChromatographyflow-modebedeterminedwithoutthenecessityformulti-anglemeasurements.ConnecttheZetasizerNanotoyourSEC—Absolutemethod,nocalibrati

4、onchromatographysystemtobeusedasan—2ndvirialcoefficientdeterminationabsolutelightscatteringdetector.Simply—Shapeestimatecapabilityconnectafterthelastdetectorinyoursystem.—Protein-ligandbindingThesizeisplottedinrealtimeasthematerialiselutedfromthecolumn.Averagesacrosseachpeakarecalculatedautomat

5、icallyattheendofthemeasurement.—DisplayasmolecularweightattheendProteinsizeofthemeasurement—Onlysecondstochangefrombatchtoflow-modeNon-invasivebackscatter(NIBS)technologytakesproteinsizemeasurementtonewlevelsof—Nocolumncalibrationsensitivitywiththepossibilityof1000Dasizeand—Determineoligomerics

6、tateofeachsub1nmdetection.ThenewZetasizerNanoiselutedpeakthechoiceforaccurate,reliableandrepeatable—Quantifypolydispersitysizeanalysisincludingproteinmeltingpoint—CompatiblewithanySECsystemdetermination.—Proteinsizeanalysis—Screeningforcrystallizationconditions—Determineaggregates—Proteinmelti

7、ngpoints2TheZetasizerNanowww.malvern.com/proteinsMakinglightworkforproteinsAtMalvernweconstantlyseektoimproveeveryinstrumentwedesignandproduce.Thisprocessismadepossiblebecausewecontinuallyaskourcustomerswhattheythinkandwhattheyneedbefor