il-33诱生的供者来源bmdc输注延长同种异体小鼠皮肤移植物生存时间及机制

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1、分类号学号M200974917学校代码10487密级硕士学位论文IL-33诱生的供者来源BMDC输注延长同种异体小鼠皮肤移植物生存时间及机制学位申请人：张宏玮学科专业：免疫学指导教师：方敏教授龚非力教授答辩日期：2012年1月AdissertationsubmittedtoHuazhongUniversityofScienceandTechnologyfortheDegreeofMasterofMedicineThemechanismsunderlyinginfusionofIL-33induceddonor-derivedBMDCprolongsmu

2、rinecutaneousallograftsurvivalCandidate:HongweiZhangMajor:ImmunologySupervisor:Prof.MinFangProf.FeiliGongHuazhongUniversityofScience&TechnologyWuhan430074,P.R.ChinaJanuary,20122独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡

3、献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。保密□，在_____年解密后适用本授权书。本论文属于不保密□。（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年

4、月3目录一、英文缩略词表·············································1二、中文摘要··················································3三、英文摘要··················································6四、前言····················································9五、第一部分············································

5、·····11六、第二部分·················································26七、讨论··················································37八、参考文献·················································38十、综述···················································45十一、附录（攻读学位期间发表论文目录）··························

6、59十二、致谢··················································60I华中科技大学硕士学位论文英文缩略词表APCAntigenpresentingcell抗原提呈细胞BMDCBonemarrowderiveddendriticcell骨髓来源的树突状细胞BFABrefeldin布雷菲德菌素CDClusterofdifferentiation分化群CFSECarboxyfluoresceindiacetate羧基荧光素二醋酸盐succinimidylester琥珀亚胺基DCdendriticcell树突状

7、细胞FBSFetalbovineserum胎牛血清FCMFlowcytometry流式细胞术FITCFluoresceinisothiocyanate异硫氰酸荧光素GM-CSFGranulocyte-macrophagecolony粒细胞-巨噬细胞集落刺激stimulatingfactor因子iDCImmaturedendriticcell未成熟的树突状细胞IFN-γInterferon-gamma干扰素-gamaILInterleukin白细胞介素LLitre升mgMilligram毫克MHCMajorhistocompatibilitycomple

8、x主要组织相容性复合体minMinute分钟mlMillilitre毫升MLCMixedlym