pdms微通道内金属微点上dn固定技术-研究

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1、重庆大学硕士学位论文英文摘要covalentbilldiIlgmaIlconventioflal．(2)WitllnegativecllargeofDNAhadbe钮adsorbedonthecathode，butnotonttle锄odeatDC弱ourexpectation，meintellS时ofnuorescencemicrodDtllasbeeniI】皿uencedwiⅡlDNAconcen仃atioIl，electrolytecoIlcenl∞atioll，microdotsize，thedistancebet、veenele咖des剐1delec仃i

2、ccoIlductiontillle．①hlthemicro—challIlelof20山【Ildiameter，novisiblenuorescencenlicrodotfomedi11廿leend．Thecrossil唱dotareaofStaj山essSteel疵wtlichdi锄ete瑙werc40，60，80and100Um、v嬲directlyproportionalt0廿1eadsorbedDNAfluorescenceintensi够②ConstantcoIlditionSinotllerc嬲es，tlleDNAconcemrationi11cre

3、嬲edgradu甜lyi11tlletin坨betwe胁O．00305-12．5姗o‰l，nuorescencei11teIls埘continuedt0iIlcrea∞诵t11me缸e90iIlgon，bmWhenDNAconcemrationw嬲moren1孤0．78125IlIIlonlllt11eirnpactionofitis锄a11．Whent11eDNAconCem瞰ionw嬲O．00305nmo№lbecau∞tlleDNAconcen臼『ationwaSsmall胁ou曲，itisdimcultiIlarelativelyshorttimet0fo

4、衄avisibleDNAnuorescencemicrodot．(蓼omerConditionSiIl廿1ecaseofacorls觚conca响眦ioll，whenNaCLconcen们tionisbe帆eentlleO．063—0．25Inol／L趾dincreased伊adumly，ⅡleadsorbedssDNAmicrodotnuorescenceiIlteIlsi够cominuedtoincre嬲e，butt11eiⅡlpactbec锄esmmlW}lenitismorem觚O．25mol／LWhentlleconc朗雠ionofNaCLiIlO．06

5、3mDⅥ。，itisdi伍cuntofbrm廿leefI’ectiveDNAnuorescenCelllicro(k，ton廿lestainlesssteelmicrodoti11nle50s．(虿hheninterelec仃odedist锄ceiIlcre嬲e也theacISI蚓bedssDNAr11icmdotnuoresCenc圮i11把【lsit)rcont证uedt0decrease，itisdimculttof．0皿alee妇f-ectiveDNAfluo陀scencemicmdoton廿1es“Jllesssteelmicrod优、)l，：henⅡl

6、eiIlterelec们dew嬲more520pm．@蛔henelectricconductionw嬲be帆e吼Ⅱle0—200s，andi11creaSed莎adually，nleadsorbedssDNA埘crodotnuorescencei11．tensit)rcontiImedtoincreaSe，inmesix廿lseconds趾dcon们llable，恤tis，avisiblenuorescentIIlicrodoth嬲beenfoⅡned，buttlleimpactbec锄esmallWllenitismorethan100s．(3)啦ercoati

7、ngalayefofPDMSontllestajnIesssteelmicrodotssuI．f．ace，p01)币)啪leh嬲be吼modifiedonnlesul_fkeofIllicrodlotthroughtheelectrochemicalmetllod，andattlles锄etiIneta唱etDNAh嬲beenilI吼obilizedtoo．触ertllat，tllecurrentclla】[19eexperimentsa_bout廿leteIn：pefa：ture跚ldhybridizationhadbeenf砬shed，tlleresultsa

8、ppe盯也