临床分离铜绿假单胞菌氨基糖苷类修饰酶耐药机制研究

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1、中图分类号：R446.5硕士学位论文临床分离铜绿假单胞菌氨基糖苷类修饰酶耐药机制研究院（系）、所临床医学院研究生姓名宋敏学科、专业内科学导师姓名王开正刘靳波二Ο一三年四月目录摘要..............................................................................................................1前言.......................................................................................

2、.......................6第一部分铜绿假单胞菌耐药情况及AMEs耐药基因检测...........................91.1材料与方法............................................................................................91.1.1实验材料与仪器..........................................................................91.1.2实验方法...............

3、.....................................................................101.2结果......................................................................................................131.2.1铜绿假单胞菌对15种抗菌药物的耐药情况.........................131.2.2AMEs基因检测结果...........................................

4、.......................141.2.3AMEs序列分析结果..................................................................141.2.4多基因聚类分析结果................................................................171.2.5AMEs基因与氨基糖苷类抗菌药物的相关性..........................171.3讨论...........................................

5、...........................................................21第二部分基于基因外重复回文序列的铜绿假单胞菌分子分型..................242.1材料与方法..........................................................................................242.1.1实验材料与仪器......................................................................

6、..242.1.2实验方法....................................................................................242.2结果......................................................................................................252.2.1REP-PCR电泳指纹图谱............................................................252

7、.2.2REP-PCR聚类分析结果............................................................262.3讨论......................................................................................................27第三部分基于蛋白指纹图谱的铜绿假单胞菌分子分型..............................293.1材料与方法...............