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1、作物学报ACTAAGRONOMICASINICA2009,35(5):861−866http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2009.00861小麦分子遗传图谱的加密11,2,**1131,*李艳秋苏志芳王立新季伟姚骥赵昌平123北京市农林科学院北京市杂交小麦工程技术研究中心,北京100097;河套大学农牧科学系,内蒙古临河
2、015000;华中农业大学生命科学技术学院,湖北武汉430070摘要:高密度的分子标记遗传图谱是QTL定位、图位克隆和分子标记辅助选择等研究的基础。以小麦品种“京花1号/小白冬麦”的双单倍体(DH)群体和“农大015/复壮30”的重组自交系(RIL)群体为作图群体,选用在DH群体双亲间的339个多态性标记和在RIL群体双亲间的343个多态性标记分析作图群体各个株系的基因型,对本中心近年开发的SCAR、EST-SSR标记以及他人开发的SSR、EST-SSR标记进行了染色体定位,并利用连锁分析软件Joi
3、nmap4.0将2个作图群体的结果整合,最终构建了10个连锁群,将217个SSR、EST-SSR和SCAR位点定位在9条染色体上,进一步提高了小麦遗传图谱的密度。关键词:小麦;SSR;EST-SSR;SCAR;遗传图谱IncreasingDensityofWheatGeneticLinkageMapwithMolecularMakers11,2,**1131,*LIYan-Qiu,SUZhi-Fang,WANGLi-Xin,JIWei,YAOJi,andZHAOChang-Ping12Beijing
4、EngineeringandTechniqueResearchCenterforHybridWheat,Beijing100097,China;DepartmentofFarming-GrazingScience,Hetao3University,Linhe015000,China;CollegeofLifeScienceandTechnology,HuazhongAgriculturalUniversity,Wuhan430070,ChinaAbstract:Highdensitygeneticl
5、inkagemapisthegroundworkformappinggeneorquantitativetraitloci,map-basedcloningandmarker-assistedselection.Toincreasethemarkerdensityongeneticlinkagemapofwheat(TriticumaestivumL.),thedoublehaploid(DH)populationderivedfromJinghua1/XiaobaiDongmaiandtherec
6、ombinantinbredlines(RILs)ofNongda015/Fuzhuang30wereusedinthisstudy.Atotalof339polymorphicmarkersbetweentheDHlinesand343polymorphicmark-ersbetweentheRILlinesweredetected.UsingtheDHpopulation,208markersweremappedon21chromosomes,covering3493.6cM;andusingt
7、heRILpopulation,299markersweremappedon34linkagegroupswiththeaveragedistanceof15.5cM.Thetwolinkagemapshad56consistentmarkersinthesimilarregionsofchromosomes.UsingJoinmap4.0software,tenlinkagegroupsfromthetwolinkagemapswereintegrated.Thislinkagemapwascom
8、posedof217markersandcovered956.2cMofwheatgenomewithanaveragedistanceof4.4cMbetweenmarkers.Theproportionofsegregationdistortionlociwas3.2–55.6%oneightchromosomes.Mostmarkersinthismaphadtheconsistentlocationswiththosementionedinpreviousre
