PTHrP对大鼠睾丸间质干细胞发育的影响及机制研究

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1、温州医科大学硕士学位论文分类号：R339.2单位代码：10343学号：151002389硕士学位论文论文题目:PTHrP对大鼠睾丸间质干细胞发育的影响及机制研究研究生姓名：宋甜甜学科专业：麻醉学类型：学术型指导教师：连庆泉教授二O一八年五月温州医科大学硕士学位论文论文题目：PTHrP对大鼠睾丸间质干细胞发育的影响及机制研究答辩委员会主席：喻田教授遵义医学院答辩委员会成员：周文华教授宁波大学医学院缪长虹教授复旦大学附属肿瘤医院论文答辩日期：2018年5月18日温州医科大学硕士学位论文目录一、中英文缩略词对照表···································

2、··1二、摘要1.中文摘要················································22.英文摘要················································4三、正文1.前言····················································72.材料和方法··············································93.结果···················································254.

3、分析和讨论·············································365.结论···················································396.参考文献···············································40四、附录··················································42五、致谢··················································43六、综述···········

4、·······································44七、学位论文独创性声明····································59温州医科大学硕士学位论文缩略词对照表英文缩写英文全名中文全名SLCStemLeydigCell睾丸间质干细胞LCLeydigCell睾丸间质细胞EDSEthanedimethanesulfonate乙烷二甲烷磺酸PTH1RParathyroidhormonereceptor1甲状旁腺激素受体1TTestosterone睾酮PTHrPParathyroidHormone-RelatedProtein甲

5、状旁腺激素相关蛋白PTHParathyroidHormone甲状旁腺激素LHLuteinizinghormone促黄体生成素LHCGRLuteinizinghormonereceptor黄体生成素受体Scarb1ScavengerreceptorclassBtypeIB类清道夫受体1Starsteroidogenicacuteregulatory类固醇合成急性调节蛋白proteinCYP11A1CytochromeP450cholesterolside细胞色素P450胆固醇侧chaincleavageenzyme链裂解酶3β-HSD13β-hydroxysteroiddehy

6、drogenase13β-羟基类固醇脱氢酶CYP17A1CytochromeP45017α-hydroxylaseP45017α羟化酶17β-HSD317β-hydroxysteroiddehydrogenase317β-羟基类固醇脱氢酶311β-HSD111β-HydroxysteroidDehydrogenase111β-羟基类固醇脱氢酶1Q-PCRreal-timePCR实时定量聚合酶链式反应Rps16primerstoribosomalproteinS1核糖体蛋白S16BCAbutyleyanoacrylate聚氰基丙烯酸正丁酯BSAAlbuminfrombovine

7、serum牛血清白蛋白DMSODimethylsulfoxide二甲基亚砜PBSphosphatebuffersaline磷酸缓冲盐溶液α-SMAα-Smoothmuscleactinα平滑肌肌动蛋白1温州医科大学硕士学位论文PTHrP对大鼠睾丸间质干细胞发育的影响及机制研究中文摘要目的：睾丸间质干细胞（StemLeydigcell，SLC）的发育受多种调节因子的影响，但目前其发育调节机制尚不清楚。大鼠SLC含高表达甲状腺旁腺激素受体1（PTH1R），一旦分化到间质细胞（Leydigcell，LC）系，