山东省芍药红斑病病原菌鉴定

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1、园艺学报，2016，43(2)：365–372.ActaHorticulturaeSinicadoi：10.16420/j.issn.0513-353x.2015-0590；http：//www.ahs.ac.cn365山东省芍药红斑病病原菌鉴定1121,*李丽，宋淑香，刘会香，郭先锋12（山东农业大学林学院，山东泰安271018；山东农业大学植物保护学院，山东泰安271018）摘要：从山东菏泽和泰安两地感病芍药（PaeonialactifloraPall.）植株的叶片上进行了红斑病病原分离纯化和致

2、病力检测，并对致病菌进行了形态学鉴定及rDNA-ITS序列分析。研究结果表明，病样组织中可分离到两种疑似病原菌，分离比例为4.8︰1，初步鉴定为链格孢（Alternariaalternata）和细极链格孢（A.tenuissima）。进一步rDNA-ITS分析结果表明，两种病原菌5.8SrDNA及其两侧ITS区序列分别与GenBank中序列号为KF380822.1、HM467832.1的同源性最高，均为99%，由此确定两种病原菌分别为链格孢Alternariaalternata和细极链格孢A.ten

3、uissima，其序列已在GenBank登录，登录号分别为KR912224和KR912225。因此，确定山东省芍药红斑病是由链格孢A.alternata和细极链格孢A.tenuissima两种病原菌引起的复合侵染。关键词：芍药；红斑病；病原鉴定；链格孢属；rDNA-ITS+中图分类号：S682.12文献标志码：A文章编号：0513-353X（2016）02-0365-08IdentificationofRedSpotPathogensonPeonyinShandongProvince1121,*LI

4、Li，SONGShu-xiang，LIUHui-xiang，andGUOXian-feng12（CollegeofForestry，ShandongAgriculturalUniversity，Tai’an，Shandong271018，China；CollegeofPlantProtection，ShandongAgriculturalUniversity，Tai’an，Shandong271018，China）Abstract：Redspothasbeenoneofthemostseriousd

5、iseasesinherbaceouspeonyproduction.Identificationofthepathogencausingthediseaseisnecessaryforthedeterminationofeffectivetreatmentoptions.Inthepresentstudy，firstly，thefungiwereisolatedandpurifiedfromtheinfectedleavesandthepathogenicitywasdetectedbyartif

6、icialinoculation.ThenthemorphologicalcharacteristicsandrDNA-ITSsequenceofthepathogenscausingredspotdiseasewasstudied.Theresultsdemonstratedthattwokindsofsuspectedfungi，AandB，wereisolatedfromthedisease-infectedsamples，withtheisolationratioof4.8︰1.Bothfu

7、ngiwerefurtherconfirmedpathogenicandthereforedeterminedasthepathogens.ThemorphologicalcharacteristicsrevealedthatthesetwopathogenswereAlternariaalternateandA.tenuissima，whichwasvalidatedbyfurtherrDNA-ITSanalysis.ITS1-5.8SrDNA-ITS2sequencesofpathogenAan

8、dpathogenBwererespectivelyhighlyhomogenicwiththoseofKF380822.1andHM467832.1inGenBankdatabase，hencetheywererespectivelyidentifiedasA.alternateandA.tenuissima.ThesequencesofthesetwopathogenshavebeensubmittedtoGenBank，andtheaccessionnumber