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1、NANOLETTERSProbingSingleDNAMoleculeTransport2004Vol.4,No.11UsingFabricatedNanopores2293-2298PengChen,†JiajunGu,‡EricBrandin,†Young-RokKim,†QiaoWang,§andDanielBranton*,†DepartmentofMolecularandCellularBiologyandDiVisionofEngineering&AppliedSciences,HarVardUniVersity,Cambridge,Massach
2、usetts02138,andDepartmentofRadioEngineering,SoutheastUniVersity,Nanjing210096,P.R.ChinaReceivedAugust19,2004;RevisedManuscriptReceivedSeptember2,2004ABSTRACTNanoporescanserveashighthroughput,single-moleculesensingdevicesthatprovideinsightintothedistributionofstaticanddynamicmolecula
3、ractivities,properties,orinteractions.WehavestudieddoublestrandedDNAelectrophoretictransportdynamicsthroughfabricatednanoporesinsiliconnitride.Afabricatedporeenablesustointerrogateabroaderrangeofmoleculeswithawiderrangeofconditionsthancanbeinvestigatedinaself-assembledproteinporeina
4、lipidmembrane.RecentexperimentsshowthatananoporeinathininsulatingdetectioninR-hemolysindependsonconductionchangesmembraneseparatingtwoionicsolutionfilledchamberscaninducedbyapolymer'spresenceinthechannel'sneck,andserveasasingle-moleculesensingdevicethatprovidesbecausethisneckisappro
5、ximately5nmlong,7theconduc-insightintothedistributionofstaticanddynamicmoleculartancechangesreflectthepresenceofupto10-15nucleotidesactivities,properties,orinteractions.1-5Asingle-moleculethatspanthisdistance.8Higherresolutionworkthatseeksapproachmakesitpossibletoexploreindividualmo
6、leculartosensefewernucleotidesatanyonetimewillprobablydetailsthatcannotbeobtainedbyensemble-averagedresultsrequireotherdetectionmodesinvolvingon-boardelectronicsandthespeedandeasewithwhichmoleculescanbecapturedthatcannotbesupportedbyalipidbilayer.andsensedbyananoporemakeitpossibleto
7、achieveveryhighthroughputsandexamineastatisticallyvalidsampleToovercomethelimitationsofaproteinporeinalipidofthemolecularpopulation.bilayer,solid-statenanoporeshavebeenfabricatedtoreplaceMostnanoporestudieshavereliedonmeasuringionicorcomplementproteinpores.9-12Here,wepresentourfirst
8、currentblockagescau
