大豆遗传图谱构建和重要性状的QTL定位

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1、摘要的表型变异；F2：3群体检测到5个QTL问互作，可解释总表型变异的61．04％，其中，效应最大的是qZG-Dlb．2和qZG．D2．J显性×显性上位性互作，为14．19，可解释47．47％的表型变异。但是，未发现相同的QTL。不过，分别位于F和O等连锁群上的qZG-F和qZG-O-2与Mian等(1998)和吴晓雷等(2001)的结果一致。3．为进一步检验和利用上述QTL检测结果，结合大豆对豆卷叶螟抗性，主茎节数、大豆对花叶病毒抗性和分枝数四性状的QTL定位结果，选择表型贡献率最大的三个QTL，计算出与这些QTL紧密连锁分子标记基因型相对应数量性状平均数和标

2、准差。结果表明，最大或最小数量性状平均数的标记基因型并不是完全来自P1(或P2)的，而是两亲本的组合。这显示QTL间存在互作，进一步证实上述已获得的结果。关键词：大豆；分子标记；花叶病毒；卷叶螟；SSR；QTL定位；遗传ⅢABSTRACTConstructionofgeneticlinkagemapandQTLmappingof‘portanttraits‘,ybean『G缈c／nemaximportanttraitsinsoyDeanctnemaxI“Zy(L．)Merrill】ABSTRACTTheconstructionofgeneticlinkagegr

3、oupsandthemappingofQTLforquality，resistanceandagronomictraitsaremaincomponentsofplantgenomicsresearch．Inthisstudy，two—hundredandfourty—fourF2plantsderivedfromasoybean(Glycinemax)CROSSbetweenLishuizhongzihuangif,1)andNannong493-1(P2)weregenotypedusing136pairsofpolymorphismsimplesequen

4、cerepeats(SSR)molecularmarkers．nemolecularmarkerdatasetwasusedtoconstructionsoybeangeneticlinkagegroupsusingJoinmapsoftware(version3．0)．Someimportanttraits，i．e．，oilandproteincontents，100-seedweight，andtheresistanceofsoybeantoBeanpyralid(LamprosemaindicataFabricius)inF2population，ther

5、esistanceofsoybeantosoybeanmosaicvirus(SMV)withstrainSaintheF2：3population，andthenumberofbranches，thenumberofmain-stemnodesandplantheightintheF2andF2：3populations，weremeasured．neobservationsforthequantitativetraitsmentionedabove，aiong谢tllthemolecularmarkerdataandthelinkagegroups，were

6、usedtomaptheircorrespondingquantitativetraitloci(QTL)usingcompositeintervalmappingandmultipleintervalmappinginthesoftwareofWindowsQTLCartographerV2．5．111emainresultswereasfollows．1．Sixty—sixSSRmarkerswereassembledintotwenty—onelinkagegroups，whichcorrespondwitllsoybeanchromosomes、析tll

7、specificmarkersinsoybeanpublicgeneticgroups．nelengthofeachlinkagegrouprangedfrom17．71cMto129．33cMandthetotallengthwas1091．34cM，withanaveragemarkerspacingof16．53eM．Thelinkagegroupwiththelargestaveragemarkerspacingof43．55cMwastheDlb，andtheonewiththesmallestaveragemarkerspacingof13．86cM

8、theM．Therewe