富硒酵母发酵优化试验与其联合丁酸梭菌制剂对肉鸡养殖上的初步应用

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1、万方数据Findingthebestfermentationconditionsofa-●一一一‘————selenium-enrichedSaccharomycescerevisiaeandtheaoolicationcombinedselenium--enrichedSaccharomycescerevisiaewithpreparationofClostridiumbutyricuminbroilerchickens1一"‘奎anlei(PreventiveVeterinaryMedicine)Directedby

2、prof．Pankang-chengAbstractThisstudywasaboutoptimizingthecultureconditionofaselenium．enrichedSaccharomycescerevisiae．Tofindthebestfermentationconditions，thePotatoDextroseMediumWasthefermentationmedium．SevenoptimizationoffactorsaboutthetimeofaddingSe，theconcentrati

3、onofSe，thecultivationtemperature，theliquidvolume，theinocultionquantity,thespeedoftableconcentrator,thecultivationtemperatureWasdesigned．Adding0．25mg／kgseleniuminselenium—enrichedSaccharomycescerevisiaeandO．1％preparationofClostridiumbutyricumtothebasicrationofbroi

4、lerchicken，ascertaintheeffectsofitspreparationonbroilerchicken’Sgrowthperformance，slaughterperformance，cecumfloraandjejunumflora，antioxidantactivity,theindexofill-ffnuneorgan，thedevelopmentofjejunumvillusandileumvillus．Theresultsofexperimentwereasfollows：1．Thestu

5、dyWasaboutfermentationconditionsofselenium-enrichedSaccharomycescerevisiae．thePotatoDextroseMediumisthefermentationmedium．SevenoptimizationoffactorsaboutthetimeofaddingSe，theconcentrationofSe，thecultivationtemperature，theliquidvolume，theinocultionquantity,thespee

6、doftableconcentrator,thecultivationtemperature．Bymeasuringthebacteriavolumeoftheselenium-enrichedSaccharomycescerevisiaet，theseleniumcontentionofselenium-enrichedSaccharomycescerevisiae，Thefollowingresults：theoptimalliquidvolumeWas40mlin250mlconicalflask，theoptim

7、alinocultionquantityis10％，theoptimalcultivationtemperaturewas34℃，theoptimalspeedoftableconcentratorwas200r／min，andaddingSeaftercultivating24hours，IIl万方数据theconcentrationofSewas50gg／ml，thefermentationcouldbefinishedin36hours．Thebiomass(dryweight)reached5．679／L，the

8、Unitofseleniumwas6511．225499／gandtotalSecontentofthecellsWas36904．17299／L．2．150one-day-oldAvainbroilerchickenswererandomlydividedintofivetreatmentsin3replicati