资源描述：
《A Root-Expressed Magnesium Transporter of the MRS2MGTMRS2_Gene Family in Arabidopsis thaliana Allows for Growth inMGT的根表达镁转运蛋白 拟南芥中的基因家族允许生长 低Mg2+环境》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、ThisarticleisaPlantCellAdvanceOnlinePublication.Thedateofitsfirstappearanceonlineistheofficialdateofpublication.Thearticlehasbeeneditedandtheauthorshavecorrectedproofs,butminorchangescouldbemadebeforethefinalversionispublished.Postingthisversiononlinereducesthetimetopublicationbyseveralweeks.ARoot-Ex
2、pressedMagnesiumTransporteroftheMRS2/MGTGeneFamilyinArabidopsisthalianaAllowsforGrowthinLow-Mg2+EnvironmentsWMichaelGebert,aKarolineMeschenmoser,aSonˇaSvidova´,bJulianWeghuber,bRudolfSchweyen,b,1KarolinEifler,aHenningLenz,aKatrinWeyand,aandVolkerKnoopa,2aInstitutfu¨rZellula¨reundMolekulareBotanik,U
3、niversita¨tBonn,D-53115Bonn,GermanybViennaBiocenter,Abteilungfu¨rMikrobiologieundGenetik,A-1030Wien,AustriaTheMRS2/MGTgenefamilyinArabidopsisthalianabelongstothesuperfamilyofCorA-MRS2-ALR-typemembraneproteins.ProteinsofthistypearecharacterizedbyaGMNtripeptidemotif(Gly-Met-Asn)attheendofthefirstoftw
4、oC-terminaltransmembranedomainsandhavebeencharacterizedasmagnesiumtransporters.Usingtherecentlyestablishedmag-fura-2systemallowingdirectmeasurementofMg2+uptakeintomitochondriaofSaccharomycescerevisiae,wefindthatallmembersoftheArabidopsisfamilycomplementthecorrespondingyeastmrs2mutant.Highlydifferen
5、tpatternsoftissue-specificexpressionwereobservedfortheMRS2/MGTfamilymembersinplanta.Sixofthemareexpressedinroottissues,indicatingapossibleinvolvementinplantmagnesiumsupplyanddistributionafteruptakefromthesoilsubstrate.HomozygousT-DNAinsertionknockoutlineswereobtainedforfourmembersoftheMRS2/MGTgenef
6、amily.Astrong,magnesium-dependentphenotypeofgrowthretardationwasfoundformrs2-7whenMg2+concentrationswereloweredto50mMinhydroponiccultures.EctopicoverexpressionofMRS2-7fromthecauliflowermosaicvirus35Spromoterresultsincomplementationandincreasedbiomassaccumulation.Greenfluorescentproteinreportergenefu
7、sionsindicatealocationofMRS2-7intheendomembranesystem.Hence,contrarytowhatisfrequentlyfoundinanalysesofplantgenefamilies,asinglegenefamilymemberknockoutresultsinastrong,environmentallydependentphenotype.INTRODUCT
