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1、FEBS19480FEBSLetters419(1997)69-75ArabidopsisthalianaclassIVchitinaseisearlyinducedduringtheinteractionwithXanthomonascampestrisLilianeB.deA.Gerhardta,GilbertoSachetto-Martinsb,MariaG.Contarinia,MarianaSandronia,RodrigodeP.Ferreiraa,VivianeM.deLimaa,MariaC.Cor
2、deiroa,DulceE.deOHveiraa,MàrciaMargis-Pinheiroa'*^LaboratòriodeGenèticaMolecularVegetal,DepartamentodeGenètica,UniversidadeFederaldoRiodeJaneiro,CEP21941-970,RiodeJaneiroCP68011,BrazilbLaboratoriodeMicropropagacàoeTransformacàodePlantas,DepartamentodeBiologiaC
3、elulareGenética/CEPUERJ,UniversidadedoEstadodoRiodeJaneiro,RiodeJaneiro,BrazilReceived28August1997;revisedversionreceived10October1997vacuolarlocalisation[6].TheclassIIIchitinasesarebifunc-AbstractEndochitinasesarewidelydistributedamonghighertionallysozyme/chi
4、tinaseenzymesanddonotshareanyse-plants,includinganumberofimportantcropspecies.Theyaregenerallyconsideredtobeinvolvedinplantdefenceagainstquencesimilaritywithplantchitinasesfromotherclasses.Thepotentialpathogens.WehaveclonedaclassIVchitinasegeneclassIVcorrespon
5、dstoagroupofextracellularchitinases{AtchitIV)fromArabidopsisthaliana.Southernblotanalysiswithtwocharacteristicdeletionsinthemaincatalyticdomainallowedthedetectionoftwocross-hybridisinggenesinthe[4].OtherproteinswithendochitinaseactivitywereisolatedA.thalianage
6、nome.AtchitIVtranscriptsaredetectedinfromtobacco[7].Becausetheysharesomesimilaritieswithseedpods,butnotinroots,inflorescencestems,leavesandbacterialexochitinases,butareunrelatedtotheplantchi-flowersofhealthyplants.Thetranscriptsaccumulatedverytinasespreviously
7、described,theyfallinanewgroup,theclassrapidlyinleavesafterinoculationwithXanthomonascampestris.Vchitinase.MaximummRNAaccumulationwasreachedonehourafterDuetotheirpotentialantimicrobialactivity,chitinaseshaveinfectionanddecreasedtoverylowlevels72hoursafterinduct
8、ion.ThisresultsuggestsaninvolvementofAtchitIVinreceivedmuchattention.Theseenzymesarepresentinalltheinitialeventsofthehypersensitivereaction.Nevertheless,A.plantsanalysedtodateandit
