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1、VIROLOGY128,407-417(1983)FusionResistanceandDecreasedInfectabilityasMajorHostCellDeterminantsofCoronavirusPersistenceLEEMIZZEN,STEVECHELEY,MEENARAO,ROBERTWOLF,ANDROBERTANDERSONDepartmentofMicrobiologyandImmundogy,UniversityofWesternOntario,Lmdm,OntarioN6A5Cl,CanadaReceivedFebruar
2、y15,1983;acceptedApril11,1989Mousehepatitisviruspersistsinculturesofasubline(designatedLM-K)ofmouseLMcellsbutproducesalyticinfectioninL-2cells.PersistenceintheLM-Kcellswasnotaccompaniedbyproductionoftsmutantsorofsolubleanti-MHVfactors.Infectiouscenterassaydemonstratedanapproximat
3、ely500-foldlowerlevelofinfectibilitybyMHVoftheLM-KcellsascomparedtoL-2cells.Onaninfectedcellbasis,productionlevelsofinfectiousprogenyandviralRNAwerecomparablebetweenthetwocelllines.Theextentofvirus-inducedcell-cellfusion,however,wasmarkedlyreducedintheLM-Kcells.Cell-mixingexperim
4、entsshowedthatbothinfectedL-2andLM-KcellshavethecapacityoffusingwithneighboringuninfectedL-2cellsbutnotwithuninfectedLM-Kcells.ThissuggeststhatthedecreasedleveloffusionobservedintheLM-Kinfectionisduenottoabsenceofviralfusionproteinatthecellsurface,butrathertoaninherentresistanceo
5、ftheLM-KcellmembranetoMHV-inducedfusion.ItisbelievedthatsuchfusionresistanceinLM-Kcellsmoderatesvirusdisseminationthroughouttheculture,therebycontributingtoastateofviruspersistence.INTRODUCTION1981;Siddelleta&1981).ComplementaryDNA(cDNA)probesarenowavailableWithinthepastfewyearsc
6、onsiderable(CheleyetuL,1981a,b;Leibowitzandprogresshasbeenmadetowardscharac-Weiss,1981)whichmaybeusedfortheterizing,inmolecularterms,someofthedetectionandanalysisofMHVRNA.eventsinvolvedinthecoronavirusrepli-Withthesenewlygainedinsightsintocationcycle.Murinecoronaviruses,ex-corona
7、virusreplication,itisnowpossibleemplifiedbymousehepatitisvirus(MHV),toinvestigateindividualparametersinthearenowknowntocontainthreemajorinfectiouscycletodeterminewhichfactorsstructuralproteins(CheleyandAnderson,playakeyroleindeterminingtheoutcome1981;Sturman,1977),ofwhichtheintra
8、-ofinfection.AnumberofstrainsofMHVvirion
