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1、ArchVirol(1989)107:171-177VrologyArchives©bySpringer-Verlag1989DurationofchallengeimmunitytocoronavirusJHMinmiceS.W.BartholdandAbigailL.SmithSectionofComparativeMedicine,YaleUniversitySchoolofMedicine,NewHaven,Connecticut,U.S.A.AcceptedJuly3,1989Summary.Thedurationofchallengeresistanceinmiceim
2、munizedwithmousehepatitisvirus(MHV)strainJHMwasexaminedasamodelofimmunitytocorona-virusinfection.GeneticallysusceptibleBALB/cByJmicewereimmu-nizedbyintranasal(i.n.)orperos(p.o.)inoculationwithMHV-JHMorsteriletissueculturefluid(sham)thenchallengedi.n.withMHV-JHMorsteriletissueculturefluid1,6,or
3、12monthslater.Fourdaysafterchallenge,virusinnasalturbinatesandliverwasquantified,andprevalanceofmicroscopiclesionsinliverandgut-associatedlymphoidtissuewastabulatedasindicesofchallengeresistance.MHV-immunizedandchallengedgroupswerecomparedtosham-immunizedandchallengedgroups.Miceimmunizedbyi.n.
4、inoculationwerestronglyresistanttochallengeat1,6,and12months.Miceimmunizedbyp.o.inoculationwereresistantat1month,butbecamepartiallysusceptibletore-infectionat6and12months,baseduponallindices.Thesedataindicatethat,dependinguponrouteofimmunization,micecanbecomesusceptibletorein-fectionwiththesam
5、ecoronavirusstrainovertime.IntroductionCoronavirusesrepresentalargefamilyofrespiratoryandentericpathogensofabroadrangeofmammalianandavianhosts.Inhumans,respiratorycorona-virusesareimplicatedasthecauseofapproximately15to20%ofcommoncolds[6,10].Thefrequencyofcoronaviruscoldssuggeststhatindividual
6、scanberepeatedlyinfectedwithcoronaviruses.Ifrepeatedinfectionstakeplaceinasingleindividual,immunityiseitherineffective,short-livedorvirusstrain-specific.ThemurinecoronavirusMHVhasbeenausefulmodelsystemtostudycoronavirus-hostinteractions.UsingMHVstrainswithprimaryrespiratorytropism,ithasbeensho
7、wnthatmicerecoveredfromani.n.inoculationofMHVresistedi.n.challengewiththehomologousMHVstrain,butwerefullysusceptibletochallengewithadifferentMHVstrainonemonthaftertheimmunizinginfection[4].Thesedatasupportthecontentionthati.n.challenge1
