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1、JournalofNeuroimmunology,32(1991)1-91©1991ElsevierSciencePublishersB.V.0165-5728/91/$03.50JNI01060Protectionofmicefromalethalcoronavirusinfectioninthecentralnervoussystembyadoptivetransferofvirus-specificTcellclonesKenjiroYamaguchi1,.,NaoakiGoto2,ShigeruKyuwa1,Masanori
2、Hayami1,..andYutakaToyoda1tDepartmentofAnimalPathology,InstituteofMedicalScience,UniversityofTokyo,Tokyo108,Japan,and2LaboratoryofVeterinaryPathology,FacultyofAgriculture,UniversityofTokyo,Tokyo113,Japan(Received5July1990)(Revised,received1September1990)(Accepted24Octo
3、ber1990)Keywords:CD4+helperTcell;CD8+cytotoxicTcell;Cellularimmunity;Centralnervoussystem;ViralinfectionSummaryTheprotectiveeffectofamousehepatitisvirustype-4(MHV-4)-specificCD8÷cytotoxicTcellcloneandaCD4÷helperTcellclonewasexaminedbytheadoptivetransferintobrainsofmice
4、lethallyinfectedwithMHV-4.Micesurvivedacuteencephalitisifmorethan5×105cellsofeithertypeofthevirus-specificTcellcloneshadbeentransferredintoH-2-matchedrecipientsby1daypost-infection.AlthoughtheadoptivetransferofbothtypesoftheTcellclonessuppressedviralgrowthandviralantig
5、en-positivecellsinthebrains,asignificantinhibitionofvirusreplicationbythecytotoxicTcellclonewasdetectedpriortothatinducedbythehelperTcellclone.Histologically,celldestructionwasprominentinthebrainsofmicewhichreceivedthecytotoxicTcellclone.Theseresultsdemonstratethatboth
6、theCD8÷cytotoxicTcellandtheCD4÷helperTcellcanprotectmicefromalethalMHV-4infectioninthecentralnervoussystem.Introductionducedcentralnervoussystem(CNS)diseaseinananimalmodel(Wegeetal.,1982;KyuwaandMousehepatitisvirustype-4(MHV-4)infectionStohlman,1990).Intracerebral(i.c.
7、)infectionwithinmiceisoneofthebestexamplesofvirus-in-MHV-4inducesanacutefatalencephalomyelitisinalmostallthelaboratorystrainsofnaivemice(StohlmanandFrelinger,1978;Knobleretal.,Addressforcorrespondence:ShigeruKyuwa,Department1981).Survivorsshowevidenceofchronicde-ofAnim
8、alPathology,InstituteofMedicalScience,UniversitymyelinationandoccasionallysufferseverehindofTokyo,Shirokanedai,Minato
