冠状病毒Coronavirusl论文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ An RT-PCR Assay for Detection of Infectious Bronchitis Coron.pdf

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1、Chapter11AnRT-PCRAssayforDetectionofInfectiousBronchitisCoronavirusSerotypesJunfengSunandShengwangLiuAbstractAvianinfectiousbronchitisvirus(IBV),achickenGammacoronavirus,isamajorpoultrypathogen,andisprobablyendemicinallregionswithintensivepoultryproduction.SinceIBVwasÞrstdescribedin1936,manyserotype

2、sandvariantsofIBVhavebeenisolatedworldwide.IBVisolatesarecapableofinfectingalargerangeofepithelialsurfacesofthechicken,involvingtherespiratory,renal,andreproductivesystems;however,theclinicalsignsareusuallynotspeciÞcfordifferentialdiagnoses.VirusisolationiscommonlyusedfordiagnosisofIBVinfection,whic

3、hwasachievedthroughpassageofclinicalmaterialsviatheallantoicrouteofembryos.Currently,moresensitivemolecularapproachesforthedetectionofavianpathogenshavebeendeveloped,includingreverse-transcriptasepolymerasechainreaction(RT-PCR)andreal-timeRT-PCR,whicharemoresuitableforuseindiagnosticlaboratories.Int

4、hischapter,wedescribeaone-stepRT-PCRwhichcanbeusedfordetectingmostofIBVserotypesintheIBV-infectedallantoicßuidandhasbeenusedroutinelyinourlaboratoriesfordetectionofIBVs.KeywordsIBV,RT-PCR,Moleculardetection,Diagnosis,Clinicalsamples1IntroductionInfectiousbronchitis(IB)isanacuteandhighlycontagiousvir

5、aldiseaseofchickencausedbytheinfectiousbronchitisvirus(IBV).Thisdiseaseisprevalentthroughouttheworldandaffectstheperformanceofbothmeat-typeandegg-layingbirds,therebycausingsevereeconomiclosswithinthepoultryindustry.IBcanaffectchickensofallagesandresultinrespiratorydisease,nephritis,proventriculitis,

6、anddecreaseineggproductionandeggquality[1Ð3].Furthermore,thisdiseaseisoftencomplicatedbysecondarybacterialinfectionsthatcauseincreasedmortality.Itmaybethemosteconomicallyimportantviralrespiratorytractdiseaseofchickensincountriesandregionswherethereisnohighlypathogenicavianinßuenzavirusorvelogenic(hi

7、ghlypathogenic)Newcastlediseasevirus[4].LeyiWang(ed.),AnimalCoronaviruses,SpringerProtocolsHandbooks,DOI10.1007/978-1-4939-3414-0_11,©SpringerScience+BusinessMediaNewYork2016121122JunfengSunandShengwa