葡萄糖标准曲线.doc

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1、[2013/3/15:24"/Graph1"(2456363)]LinearRegressionthroughoriginforData1_B:Y=B*XParameterValueError------------------------------------------------------------A0--B0.802030.01276------------------------------------------------------------RSDNP---------------------------

2、---------------------------------0.99820.036469<0.0001------------------------------------------------------------氮源种类lsd=t0.05*sx1-x2sx1-x2=N=3L0.05=45.877L0.01=65.25334氮源用量L0.05=57.9393L0.01=82.4102硫酸铵和豆饼粉含量L0.05=36.0044L0.01=51.211硫酸铵和蛋白胨含量L0.05=38.3182L0.01=54.50

3、201β-葡萄糖苷酶CxC111结晶纤维素无定形纤维素纤维二糖葡萄糖纤维二糖EGI、EGII纤维糊精CBHI、CBHIICBHI、CBHII纤维素BG葡萄糖实验号因素酶活值（U/g）ABCDⅠⅡⅢ平均值11111276.502259.698291.778275.99221222301.122251.622348.146300.29731333267.357253.553244.109255.00642123238.445248.778294.082260.43552231332.598291.779302.362308.9136

4、2312288.606398.204405.511364.10773132377.461383.599329.895363.65283213444.975436.250438.841440.02293321477.430446.196423.886449.171K1831.296900.0811080.1220T=3017.599K2933.4561049.2331009.9040K31252.8461068.285927.5720277.098300.026360.0410311.152349.744336.6340417.6

5、15356.095309.1910R140.51656.06850.8490（2）正交实验结果表16正交实验结果及分析Table16.Resultsandanalysisoforthogonaltest实验号因素酶活值（U/g）ABCDⅠⅡⅢ平均值11111276.502259.698291.778275.99221222301.122251.622348.146300.29731333267.357253.553244.109255.00642123238.445248.778294.082260.43552231332.59

6、8291.779302.362308.91362312288.606398.204405.511364.10773132377.461383.599329.895363.65283213444.975436.250438.841440.02293321477.430446.196423.886449.171K1831.296900.0811080.1220T=3017.599K2933.4561049.2331009.9040K31252.8461068.285927.5720277.098300.026360.0410311.

7、152349.744336.6340417.615356.095309.1910R140.51656.06850.8490本项目以绿色木霉为菌种，对绿色木霉产纤维素酶固态发酵培养基配方中氮源进行选择优化，提高产纤维素酶的活力。采用单因素实验分别研究了培养基生长氮源种类、生长氮源用量对产纤维素酶活力的影响，再通过研究培养基中有机氮源和无机氮源的不同组合对产纤维素酶活力的影响，最后通过正交实验三种不同氮源组合对培养基配方中氮源进行了优化，以确定最优氮源组合。实验结果表明培养基配方中最优氮源组合为：硫酸铵为1.2%，豆饼粉为0.4%，

8、蛋白胨为0.4%。采用此培养基中最优氮源组合固态发酵培养绿色木霉产纤维素酶活力达到448.558U/g。Theprojectiswithgreentrichodermaviridestrain,forgreentrichodermavirideproducesc