脑脊液引流对颅脑创伤后免疫损伤的影响.pdf

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1、天津医药2012年1月第4O卷第1期57doi：lO．3969／j．issn．0253-9896．2012．01．020脑脊液引流对颅脑创伤后免疫损伤的影响张洪伟闫华武俏丽张国斌刘暌只达石摘要目的：探讨脑脊液引流对颅脑创伤后血清抗脑抗体及白细胞介素(IL)一18水平的影响。方法：32只大白兔随机分为对照组和脑脊液引流组(引流组)，每组16只。脑损伤后0～7d每天取患兔脑脊液1mL，对照组将脑脊液回注入脑池；引流组弃去脑脊液。2组均于创伤后第1⋯3714、21天取血2mL，分离血清，采用酶联免疫吸附试验(ELI．

2、SA)检测IL一1B和抗脑抗体(ABAb)水平。结果：创伤后对照组ABAb水平逐渐增高，第l4天达高峰，之后下降；引流组伤后第1天ABAb水平最高，之后逐渐降低。除第1天外，其他各时点的ABAb水平引流组均低于对照组(P

3、键词颅脑损伤脑脊髓液分流术抗脑抗体白细胞介素lB兔EfectsofCerebrospinalFluidDrainageonChangesofAntibrain—AntibOdyandInterleukin-113inRabbitswithTraumaticBrainInjuryZHANGHongwei，YANHua，WUQiaoli，ZHANGGuobin，LIUKui，ZHIDashiDepartmentofNeurosurgery,TheSecondHospitalofTianfinMedicalUnive

4、rsity,Tianjin300211，ChinaAbstractObjective：Tostudytheprotectiveeffectofcerebrospinalfluiddrainagetherapyonimmuneinjuryinrabbittraumaticbraininjury(TBI)models．Methods：Thirty-tworabbitswererandomlydividedintotwogroupsincluding16incon-trolgroupand16incerebrospi

5、nalfluid(CSF)drainedgroup．From0d-7d，rabbitsincontrolgroupweredrainedCSFandin—jectedbackintocistern，andrabbitsinCSFdrainedgroupweredrainedCSFonly．Bloodsamplesof2mLwerecollectedat1d，3d，7d，14dand21dafterTBIintwogroups．Thelevelsofinterleukin(IL)-1pandanti-braint

6、issueantibody(ABAb)weredetectedbyELISA．Results：ThelevelofABAbwasgraduallyincreasedandreachedthepeaklevelon14day，andthendecreasedafterTBIincontrolgroup．ThelevelofABAbwasthehighestonthefirstdayandthengraduallydecreasedinCSFdrainedgroup．Inadditiontothefirstday，

7、levelsofABAbwerelowerinCSFdrainedgroupthanthoseincontrolgroup<0．01)．LevelsofIL一113reachedthepeaklevelonthefirstdayandthengraduallydecreasedafterTBIinbothgroups．Inadditiontothefirstday，levelsofIL-I~werelowerinCSFdrainedgroupthanthoseincontrolgroup

8、prove—mentratewashigherandthemortalityratewaslowerinCSFdrainedgroupthanthoseofcontrolgroup<0．05)．Conclu-sion：Thedrainageofcerebrospinalfluidcanhelptodecreaselevelsofinflammatoryfactors，whichshow