青钱柳叶总三萜刺激3T3－L1脂肪细胞的葡萄糖消耗-论文.pdf

《青钱柳叶总三萜刺激3T3－L1脂肪细胞的葡萄糖消耗-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、现代食品科技ModernFoodScienceandTechnology2014，Vo1．30，No．8StimulationofGlucoseConsumptionin3T3—-L1AdipocytesbyTriterpenoidsfromCyclocaryapaliurusLeavesFUXiao，YINZhong-ping，SHANGGUANXin．chen，PENGDa-yong，CHENJi-guang，JIANGYan2(1．JiangxiKeyLaboratoryofNaturalProductsandFunctional

2、Food，JiangxiAgriculturalUniversity，Nanchang330045，China)(2．FoodScienceandEngineeringCollege，JiangxiAgriculturalUniversity，Nanchang330045，China)Abstract：CyclocaryapaliurusisauniqueplantnativetoChina，teamadefromtheleavesofthisplantknownforitshypoglycaemicefects．Tofurtherex

3、ploreitshypoglycaemicproperty，totaltriterpenoidsfromCyclocaryapaliurusleaves(CPTT)wereextractedbyanultrasound．assistedmethodandsuccessivelypurifiedbyextraction，macroporousresinadsorption，anddecolorization．ThetotaltriterpenoidcontentofCP1wasashi}ghas86．3％．Thecontentofurso

4、licacid．oleanolicacid,maslinicacid,corosolicacid,andbetulinicacidinCPTTwere19．35％，17．38％，7．53％，4．59％，and1．42％，respectively．Noobviouschangeinglucoseconsumptionwasobservedwhen3T3·L1pre—adipocytesweretreatedwithCPTTin也ebasalcondition．buttheconsumptionsignificantlyincreaseda

5、ftersimultaneousstimulationwith10nMinsulin．Formatureadipocytes．CPTTincreasedglucoseconsumptionsignificantlyinbothbasalandinsulin-stimulatedconditions．Fordexamethasone—inducedinsulin—resistant3T3一L1adipocytes，CPTTefectivelyimprovedinsulinsensitivityandenhancedglucoseconsu

6、mptioninadose．dependentmanner,withtheoptimalefectobservedat25~g／mL．TheseresultssuggestthattriterpenoidsfromCyclocaryapaliurusleavescanefectivelyincreaseglucoseconsumptioninadipocytesandthereforehaveapotentialapplicationinboththefoodandpharmaceuticalindustries．Keywords：Cy

7、clocaryapaliurus；glucoseconsumption；insulin-resistant；triterpenoid；3T3·L1adipocytesArticleNo．：1673．9078(2014)8—3l一37、青钱柳叶总三萜刺激3T3-L1脂肪细胞的葡萄糖消耗付晓’，尹忠平’，上官新晨，彭大勇’，陈继光，蒋艳(1．江西省天然产物与功能食品重点实验室，江西南昌330045)(2．江西农业大学食品科学与工程学院，江西南昌330045)摘要：青钱柳为我国特有植物，以其叶制成的茶叶具有较好的降血糖效果。为了进一步研究其降

8、糖作用，以超声辅助法提取青钱柳叶总三萜，经萃取、大孔树脂吸附、脱色等纯化后纯度达86．3％，其中熊果酸、齐墩果酸、山楂酸、科罗索酸和白桦脂酸的含量分别为19．35％、17．38％、7．53％、4．59％和1