罗汉果组培苗二次脱毒技术探究

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1、罗汉果组培苗二次脱毒技术探究　　摘要：对罗汉果（Siraitiagrosvenorii）组培苗采用热处理和剥取茎尖技术进行二次脱毒处理，脱毒效果采用间接酶联免疫法和电镜观察法进行检测。结果表明，适合材料热处理的培养基为MS+0.3mg/L6-BA+NAA0.1mg/L+0.1mg/LIBA，热处理材料的顶芽呈锥形，利于剥取茎尖；茎尖分化最佳培养基为MS+0.3mg/L6-BA+0.05mg/LNAA+0.1mg/LGA3；38.5℃热处理10d剥取长1.0～1.5mm的茎尖进行二次脱毒培养，存活率为80.00%，脱毒率为100.00%。关键词：罗汉果（Siraitiag

2、rosvenorii）花叶病毒；茎尖；热处理；脱毒；酶联免疫法中图分类号：S668.9文献标识码：A文章编号：0439-8114（2013）05-1185-03StudyonDuplicateVirus-freeTechniquesofSiraitiagrosvenoriiTissueCultureSeedlingWUQun-ying1，LIBo-lin2，LIJing-yun3（1.BiotechnologyCollege，GuilinMedicalUniversity，Guilin541004，Guangxi，China；2.CollegeofLifeScience

3、，GuangxiNormal2University，Guilin541004，Guangxi，China；3.CollegeofBasicMedicine，GuangxiUniversityofChineseMedicine，Nanning530001，China）Abstract：ThetissuecultureseedlingsofSiraitiagrosvenoriiweretreatedbyheattreatmentandtip-strippingtoobtainvirus-freeseedling.ELISA（enzyme-linked-immunosorbe

4、ntserologicassay）methodandelectronmicroscopemethodwereappliedforvirusdetection.TheresultsshowedthatthesuitablemediumforheattreatmentwasMS+0.3mg/L6-BA+0.1mg/LNAA+0.1mg/LIBA.Terminalbudafterheattreatmentwasconicalandeasyforstrippingstemtip.TheoptimummediumforinducementoftipswasMS+0.3mg/L6-

5、BA+0.05mg/LNAA+0.1mg/LGA3.Heattreatmentfor10dat38.5℃twice，strippingshoot-tipfor1.0～1.5mmlong，thesurvivalratewas80.00%；andthedetoxicationratewas100.00%.Keywords：Siraitiagrosvenoriivirus（WMV-2-Luo）；shoot-tip；heattreatment；virus-free；ELISA2