production of 2-deoxyribose 5-phosphate from fructose to demonstrate a

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1、JournalofBiotechnology148(2010)204–207ContentslistsavailableatScienceDirectJournalofBiotechnologyjournalhomepage:www.elsevier.com/locate/jbiotecShortcommunicationProductionof2-deoxyribose5-phosphatefromfructosetodemonstrateapotentialofartificialbio-syntheticpathwayusingthermophili

2、cenzymesKohsukeHondaa,∗,ShoheiMayaa,TakeshiOmasaa,RyuichiHirotab,AkioKurodab,HisaoOhtakeaaDepartmentofBiotechnology,GraduateSchoolofEngineering,OsakaUniversity,2-1Yamadaoka,Suita,Osaka565-0871,JapanbDepartmentofMolecularBiotechnology,HiroshimaUniversity,1-3-1Kagamiyama,Higashi-Hi

3、roshima,Hiroshima739-8530,JapanarticleinfoabstractArticlehistory:SixthermophilicenzymesfromThermusthermophiluswereusedtoconstructan‘artificialbio-syntheticReceived6April2010pathway’fortheproductionof2-deoxyribose5-phosphatefromfructose.ByasimpleoperationusingReceivedinrevisedform2

4、5May2010sixrecombinantEscherichiacolistrainsproducingthethermophilicenzymes,respectively,fructosewasAccepted7June2010convertedto2-deoxyribose5-phosphatewithamolaryieldof55%.©2010ElsevierB.V.Allrightsreserved.Keywords:ThermophilicenzymeArtificialbio-syntheticpathway2-Deoxyribose5-p

5、hosphate2-DeoxyriboaldolaseThermophilicenzymesaregettingincreaseduseinbio-synthesisof2-deoxyribose5-phosphate(DR5P)usingareverseindustrialprocesses(Coolbearetal.,1992;Persidis,1998;NiehausreactionofDR5Pcleavagecatalyzedbythe2-deoxyriboaldolaseetal.,1999;Eichler,2001).Theirexcelle

6、ntstabilityandhighreac-(DERA)ofKlebsiellapneumonia(Ogawaetal.,2003;Horinouchitiontemperaturehaveadvantages,suchaseasymixing,abetteretal.,2003).Theyalsoachievedthedirectconversionofglucosesubstratesolubility,ahighmasstransferrate,andthelowrisktoDR5Pwithamolaryieldof22.1%byintegrat

7、ingDERAandofcontamination(Turneretal.,2007).RecombinantDNAtech-glycolysisofbaker’syeast(Horinouchietal.,2006a,b).DR5Pcanniqueshaveallowedtheoverproductionofthermophilicenzymesbefurtherconvertedto2-deoxyinosinebyphosphopentomutase-inmesophilicorganisms(e.g.Escherichiacoli).Useofr

8、ecombinantandnucleosidephosphorylase-cat