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1、中国科技论文在线http://www.paper.edu.cnMitochondrialgenomesequencingofthreefireflybeetles#andphylogeneticanalysisof18speciesofbeetles**5WANGKai,HONGWei,JIAOHengwu,MUFeng-Juan,ZHAOHuabin(CollegeofLifeSciences,WuhanUniversity,Wuhan430072)Abstract:Tovalidatethephylogeneticinferencebasedontr
2、anscriptomedata,weemployedthetraditionalSangersequencingtoobtaintwocompletemitogenomes(AquaticafictaandA.wuhana)andonenearlycompletemitogenome(Lamprigerayunnana);wealsoidentifiedthe13proteincodinggenes10(PCGs)inmitogenomesfromeachofthefourwhole-bodytranscriptomes;andweadditionall
3、yretrieved12publishedcompletemitogenomesfrommembersofRhagophthalmidae,Lampyridae,Phengodidae,Elateridae,Lycidae,Cantharidae,andTenebrionidae.OurmitogenomeanalysisconfirmedthedistinctivenessofRhagophthalmidaefromLampyridae,whichisconsistenttoourearliertranscriptomeanalysis.15Keywo
4、rds:Lampyridae,Phylogenomics,Mitochondrialgenome,Mitogenome0IntroductionTheoriginandevolutionofbioluminescenceinbeetleshaspromptedseveralstudiestoilluminate[1-8]thephylogeneticrelationshipswithinbioluminescentbeetles.However,thephylogenetic20positionofRhagophthalmidaehasbeenalong
5、-standingpuzzle.Therearethreemajorhypothesesconcerningthisissue.First,RhagophthalmidaehadbeenassignedtothefamilyPhengodidae[3]inferredfrommorphologicaldata.Second,RhagophthalmidaehadbeenconsideredtobeasubfamilyorgenusinthefamilyLampyridaebasedonmorphological,embryologicaland[4,6,
6、7,9]molecularevidence.Third,morerecentevidencefrombothmorphologicalandmolecular25datahassupportedthefamilialstatusofRhagophthalmidae,whichisdistinctfromboth[1,2,5,8]LampyridaeandPhengodidae.Giventhattheevolutionaryhistoryofmorphologicalfeaturesisusuallycomplex,molecularevidenceha
7、sbeenexpectedtoresolvetaxonomicstatusand[10]phylogeneticrelationshipsthathavebeencontentiousbasedonmorphologicalevidence.However,theincongruenceofmolecularevidencehasbeenobservedinphylogeneticanalyses[1,5,7]30concerningthephylogeneticpositionofRhagophthalmidae,possiblybecauseasin
8、gleorafew[11]geneslacksufficientphylogen
