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1、替米沙坦对糖尿病大鼠肝细胞生长因子的影响王小华,殷士良,赵玉萍,刘晓峰,赵春艳,周凤娟河北省唐山市滦县人民医院内分泌科河北滦县063700摘要:目的探讨替米沙坦对糖尿病大鼠肝细胞生长因子的影响。方法雄性SD大鼠40只,单纯随机分为:正常组(C组),糖尿病模型组(D组),替米沙坦干预组(T组)。D和T组高糖高脂饲料喂养4周后以25mg/kg链脲佐菌素(STZ)尾静脉一次性注射,C组常规饲料喂养4周后以等剂量的枸橼酸盐尾静脉注射。给予STZ后一周,T组给予替米沙坦8.34mg/kg/d,C和D组等剂量温水灌胃。所有大鼠继续原饲料喂养,给药l2周末结束实验。
2、双抗体夹心ELISA法和westernblot法分别检测血清和心肌中HGF的表达。结果血清和心肌中HGF水平D组,T组高于C组,T组高于D组。结论替米沙坦能够增加糖尿病大鼠HGF的表达。关键词:2型糖尿病;心肌;肝细胞生长因子;替米沙坦EffectsoftelmisartantoExpressionofhepatocytegrowthfactorintype2diabeticratsWangxiaohua,Yinshiliang,Zhaoyuping,Liuxiaofeng,Zhaochunyan,ZhoufengjuanDept.ofEndocrin
3、ology,PeopleHospital,LuanCounty,TangshanCity,HebeiProvince,Luanxian063700,China王小华,女,河北省唐山市滦县人民医院,硕士。通讯地址:河北省唐山市滦县人民医院内分泌科,063700,联系电话:13582873091,E-mail:wangxiaohua.1996@163.comAbstract:ObjectiveToexploretheeffectsoftelmisartantohepatocytegrowthfactor(HGF)intype2diabeticrats.Me
4、thodFortymaleSDratswererandomlydividedintothenormalgroup(Cgroup),diabetesmodelgroup(Dgroup),telmisartaninterventiongroup(Tgroup).RatsofDandTgroupswereinjectedbythetailveinwithstreptozotocin(STZ)25mg/kgafter4-weekfedwithhighcaloriediet;Cgroupwereinjectedbythetailveinwiththesamevo
5、lumecitricacidbufferafter4-weekfedwithcommondiet.After1weekinjectedtheSTZ,ratsofTgroupweregivenwithtelmisartan8.34mg/kg/dandratsofC,Dgroupswiththesamevolumewater.Theexperimentendeduntill2weeksgiventelmisartan.BloodsampleswereobtainedtomeasurethelevelsofHGFbydouble-antibodysandwi
6、chELISAmethod,ThesemiquantitativeexpressionsofHGFproteinofmyocardiumweredeterminedbywesternblotmethod.ResultsThelevelsofHGFofserumandmyocardiumofD,TgroupswereevatedstatisicallysignificantcomparedwihtCgroup;ThelevelsofHGFofTgroupswereevatedstatisicallycomparedwihtDgroup.Conclutio
7、nTelmisartancanincreateexpressionofHGFintype2diabeticrats.Keywords:Type2diabetesmellitus;Myocardium;Hepatocytegrowthfactor;Telmisartan糖尿病心肌病变是糖尿病慢性并发症之一,独立于冠状动脉粥样硬化之外,以微血管为主要病理改变的心肌病变[1]。可能与心肌细胞能量代谢紊乱,细胞功能改变,凋亡增加,促使心肌纤维化的一些细胞因子和炎症因子异常表达有关。HGF通过抗凋亡,促血管新生,修复内皮细胞等方面对心肌具有保护作用。但提高心肌中
8、HGF的表达,目前多采用外源性注射,由于其半衰期较短且价格昂贵,在治疗方案的选择上仍然存在较大