季节性流感监测中病毒分子变异分析的意义的论文

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1、季节性流感监测中病毒分子变异分析的意义的论文季节性流感监测中病毒分子变异分析的意义的论文【摘要】目的探讨季节性流感监测的同时，针对人群中循环的优势毒株开展基因变异分析的意义。方法以201X年深圳市流感监测系统累积的数据为基础，按周分析不同监测医院流感样病例占门诊和急诊就诊者的比例，并对病毒型别进行常规分离鉴定，对优势毒株扩增ha1区基因片段，并进行基因变异分析。结果深圳市流感样病例201X年流感样病例(ili)比例为6.67%,以低年龄为高发人群;按市、区医院和社区健康服务中心等分别分析,ili的比例按周分布一致，呈现明显的夏

2、季高峰，最活跃季节在5～7月;ili暴发疫情比散发病例提早2周出现。流感病毒分离率为11.63%,优势株为h3n2亚型(占59.5%)，b型次之(占37.0%)。h3n2亚型流感病毒ha1基因进化分析分成两支，who当年疫苗株和我国代表株仅与1～4月毒株接近，而与5月之后分离株产生了遗传距离，虽然还不能确定新的变异株出现，但提示a/sz/9/201X和a/sz122/201X两个分离株值得高度关注。b型流感病毒ha1基因进化分析发现，201X年以yamagata系最活跃。结论季节性流感监测中开展病毒分子变异分析，可以进一步查明

3、人群中所发生的优势毒株的特性，便于流行病学综合分析。【关键词】流感;人群监测;基因;变异(遗传学)epidemiologicalvalueofmolecularvariationanalysisonseasonalinfluenzasurveillancezhangshunxiang1,guliniu1，2,chengxiaowen1,hejianfan1,hudongsheng2,lvxing1,wuchunli1,lujianhua1,fangshisong1.1.shenzhencenterfordise

4、asecontrolandprevention,shenzhen518020,china;2.schoolofpublichealth,universityofzhengzhou,zhengzhou450001,china【abstract】objectivetodeterminetheepidemiologicalvalueofmolecularvariationanalysisforthepredominantstrainswiththeseasonalinfluenzasurveillance.methodspercen

5、tagesofvisitsforinfluenzalikeillness(%ili)hadbeenanalyzedasanindicatorofinfluenzavirusactivityinthemunity.nasopharyngealswabsfromthepatientswithiliwerepropagatedandisolatedforviruses.theha1regionofthepredominantvirusesweretestedbyrtpcrandsequencedsubsequently.the

6、analysesofpairwiseaminoacidvariations,geneticclustering,andphylogeneticswereperformed.resultstotalof%iliwas6.67%andtherewasasummerpeakfrommaytojuly.totalof50sporadicoutbreaksofinfluenzawereinvestigatedandthepeakoftheoutbreaksprecededthepeakof%ilibyapproximatelytwowe

7、eks.theannuallyisolatingratesofinfluenzaviruswastotally11.63%andfellbelowthepeakof%iliabout4weeks.thepredominantstrainsincludedsubtypeh3n2(59.5%)andtypeb(37.0%).phylogeneticclusteringanalysisofinfluenzah3n2virusrevealedthattherewere2branches,includingthevirusesisola

8、tedfromjanuarytoapril,andothermonthsof201X,respectively.thevaccinestrainesremendedbywhoandrepresentativestrainsremendedbyccdcwereclustered