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1、复合因素构建大鼠肝硬化模型与益舒软肝丸的干预中国组织工程研究与临床康复第『7蕾第47期2007—11—25出版JournalofClinicslRehabilitativeTissueEngineeringResearchNovember25,2007Vol11,No47基础医学复合因素构建大鼠肝硬化模型与益舒软肝丸的干预★陈超,王希君EffectofYishuruanganwanonreversingcirrhosisinratsAbstractAlia:ToinvestigatetheeffectofYishuruanganwan(YS)ontheliverfunctionand
2、histomorphologydiversityoflivercirn1osisrats.METHIODS:ThisexperimentwasfinishedinLaboratoryofTraditionaIChineseMedicinePharmacologyfNationalLeveIB)atThreeGo)rglesUniversityfr0mC)ctober2004toApri~2006.)Eightyratsweredi'idedlntocontroIlgroup(n=8)andmodellgroup(n=72)randomly.Tworatsweresacrificedo
3、nthesixthweektoensurethemodellsuccess.Attheendofinducingmodel,30ratsdiedintheprocess.Theremaining40ratswereseparatedinto4groupsrandomly:modeIcontrolgroup(n=12),YSO.4g/kggroup(n=1O),YS0.8g/kggroup(n=9),andYS1.6g/kggroup(n=9).②Thefivercirmosismodel1wasconstructedbycomplexfactorsofcarbontetrachlor
4、ideandhigh--fatIow-proteindiet.Atthesametime,salinewasusedtosupplymodelcontroIgroupbyintragastricadministration(1mLeveryrat).WhiletheratsoftreatcontrollgroupswereintragastricallyadministratedwithYSsuspension,onceperdayfor12weekscontinuously.(Food,dnnk,activity,furcoloranddeathofratswereobserved
5、.AnimaImodel'sweight,Iiverandspleenwetweightweremeasuredthentheliverandspleencoefficientswerecalculatedattheendofexperiment,respectively.PartofserumwastakentoobservecorrelativeindiceswithliverfunctionthroughautomaticbiochemicaIanalyzer.Amotherpartofserumwastakentomeasurehyaluronicacid(HA),lamin
6、in(LN)andprecollagenⅢamJnqlotterminalpeptide(PⅢNP).LefthepaticIobewasOlbbtminedtopreparetheseriaIsections.whichwereusedfolrhematoxylin.eosindyeingandVGdyeingtoobservehistologydiversity.RESIIITS:)Grossobservation:Ratsofnorm~IcontroIgroupwerealIsurvivaIandnurturedwelI.R.atsinmodelcontroIgroupwere
7、furrough,emaciated,depressed,dulled,irritableEndeasilybledontoenaiIapart.Therewere4,3,2,2deadinthemodelcontrolgroup,YSO.4g/kggroup,YS0.8g/kggroupandYS16g/kggroup,respectively.②Weight,liverandspleencoefficien~[s:Theweightofratsinmo
