伊达拉奉对新生小鼠缺氧缺血性脑损伤的保护作用

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1、伊达拉奉对新生小鼠缺氧缺血性脑损伤的保护作用?474??EnglishOriginalArticle'辄N啭刍第22卷第6期20o7年3月JApplClinPediatr.VoL22No.6.Mar.2007Neur0pr0tectiVeEffectsofEdaravoneonNeonatalMicewithHypoxia——IschemiaBrainDamageHUANGzhi一}饨,zHUChang—lian,WANGXiao—yⅡng(DepartmentofPediatrics,theThirdAffiliatedHospitalofZhengzhouUniversity,Zheng

2、zhou450052,China)Abstract:ObjectiveToevaluatetheneuroprotectiveeffectandpossiblemechanismsofedaravone(3一methyl—l—phenyl一2一pyrazolin一5一one)inneonatalHarlequin(Hq)mutantmicebrainafterhypoxia—ischemiabraininjury(HIBD)insult.MethodsThenine—day一0ldmaleHqmutantmousepupswereassignedrandomlyeitheredaravone

3、(n=16)andvehicle(rt=17)treatmentgroup.TheHqmiceweresubjectedtoleftcommoncarotidarteryocclusioncombinedwithinhalationlO0mL/Loxygenfor45minutes.Themicewerein—jectedintraperitoneallyeitherwithedaravone(10ms/kg)orequivalentvolumeofsalineimmediatelyafterarteryocclusionandafterhypoxia.Nitrotyrosineandlip

4、idperoxidationformationwereevaluatedat3hand24hafterhypoxia—ischemia(HI)byusingimmunohistochemistrystaining.Nitrotyrosineformationandcaspasesactivationwereeva/uatedeitherbyimmunoblottingorfluorogenicactivitymeasurementat24hafterHI.Braininjurywasevaluatedat72hbyneumpath0l0calSCOreandcalculatingtheinf

5、arctvolume.ResultsBraininjuryencompassedcortex,hippocampus,striatumandthalamus.Edaravonetreatmentreducedbraininjurysignificantlyinallthebrainregions.111etotalinfarctvolumewasreduced52.8%inedaravonetreatmentgroupcomparedwithvehiclegroup(P<0.001).111eedaravonetreatmentreducednitro-tyrosineformatio

6、naswellaslipidperoxidationformationsignificantly.butwithoutobviouslyeffectoncaspasesactivation.ConclusionEdara-voneaffordsneuroprotectionafterneonatalHIinsuh.whichcorrelatedwiththereductionoffreeradicalformation.JApplClinPedlatr,2007.22(6):474—478Keywords:neuroproteetion;edaravone;freeradical;hypox

7、ia—ischemia,brain伊达拉奉对新生小鼠缺氧缺血性脑损伤的保护作用黄志恒,朱长连,王小阳(郑州大学第三附属医院儿科,郑州450052)摘要:目的研究伊达拉奉(3一甲基-l苯基-2一吡唑啉-5一酮)对新生Harlequin(Hq)小鼠缺氧缺血性脑损伤(HIBD)的神经保护作用及可能机制.方法新生9日龄雄性Hq小鼠随机分为伊达拉奉治疗组(rt=16)与对照组(rt=17).小鼠在动脉结扎缺氧后分别即刻