[经典]新生大鼠缺氧缺血性脑损伤AQP-4表达及依达拉奉治疗效果的研究.doc

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1、［经典］新生大鼠缺氧缺血性脑损伤AQP-4表达及依达拉奉治疗效果的研究新生大鼠缺氧缺血性脑损伤AQP-4表达及依达拉奉治疗效果的研究［摘要］目的:探讨新生大鼠缺氧缺血性脑损伤(hypoxic-ischemicbraindamage,HIBD)后脑组织水通道蛋白-4(AQP-4)的表达变化及依达拉奉(edaravone)对其治疗效果的影响。方法:将7日龄Wistar大鼠120只随机分为3组:假手术组、缺氧缺血模型组(HIBD组)及依达拉奉治疗组,每组各40只。每组根据处死的时间不同乂分5个亚组:6、24h、3、5、7d组，每亚组各8

2、只，各组分别于不同时间点处死动物，测脑组织含水量，并采用免疫组化方法检测AQP-4的表达。结果:AQP-4在缺氧缺血6h即增强,3d达高峰,5~7d逐渐降低(P<0.01);治疗组各时间点AQP-4表达水平较HIBD组明显降低(P<0.01)o结论:AQP-4参与了HIBD脑水肿的发生发展过程，依达拉奉可降低AQP-4的表达，从而减轻脑水肿，这可能是其脑保护作用机制之一。［关键词］依达拉奉;水通道蛋白4;新生大鼠;缺氧缺血;脑水肿［中图分类号］R-33［文献标识码］A［文章编号］1674-4721(2010)03(a)-004-0

3、3ExpressionofAQP-4andeffectsofedaravoneinneonatalratswithhypoxic-ischemicbraindamageXIALi,YINHuaiqing*,YINChongjuan,WUShirun,CHANGXia,ZHANGSufen(DepartmentofPediatrics,FirstHospitalofShanxiMedicalUniversity,Taiyuan030001,China.)［Abstract］Objective:Toprobeintotheexpres

4、sionofAQP-4inbraintissueafterhypoxic-ischemicbraindamage(HIBD)inneonatalratsandtheeffectofEdaravoneonthetreatmentofHIBD.Methods:Atotalof120seven-day-oldWistarratswererandomlydividedintothreegroups(n=40),sham-operatedgroup,hypoxic-ischemicbraindamage(HIBD)groupandEdara

5、vone-treatedgroup.Eachgroupweredividedintofivesub-groups(n=8ineachsubgroup)basedondifferenttimepointsafterHIBD(6,24h,3,5,7d).Animalsweresacrificedatdifferenttimepoints.Changesofbrainwatercontentweredetermined.Andimmunohistochemicaltechniquewasusedtodeterminetheexpress

6、ionofAQP-4.Results:TheexpressionofAQP-4increasedsignificantlyon6hafterHIBD,thepeakreachedon3dand5-7dgraduallydecreased(P<0.01).ThelevelofAQP-4expressionintreatmentgroupwassignificantlylowerthanHIBDgroupateachtimepoint(P<0.01).Conclusion:AQP一4involvesintheoccurrenceand

7、developmentprocessofcerebraledemaafterHIBD.EdaravonecanreducetheexpressionofAQP-4,soastoalleviatebrainedema,whichmaybetheoneofthemechanismsofbrainprotection.［Keywords］Edaravone;Aquaporin-4;Neonatalrats;Hypoxic-ischemic;Brainedema新生儿缺氧缺血性脑病(hypoxic-ischemicencephalopat

8、hy,HIE)是由于各种围生期窒息导致胎儿或新生儿脑缺氧缺血性损伤，而脑水肿是新生儿HIE早期出现的病理生理过程，与许多后遗症的发生密切相关。近年来人们发现水通道蛋白4(AQP-4)与脑水肿的形成关系密切,而且在缺氧缺血的脑组织中AQP-4表达显著