凝集素与木霉生防菌的筛选

凝集素与木霉生防菌的筛选

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时间:2018-07-12

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1、YangHetong,TangWenhua,MaartenRyder,LiJishun,GuoYong,ZhouHongzi.2004.LectinsoffungalpathogensaspotentialtoolsinselectingpromisingbiologicalcontrolagentsofTrichodermaspp.J.ShandongUniversityofTechnology(Sci&Tech),18(6):15-23Lectinsoffungalpathogensaspotentialtoolsinselectingpromisingbiologicalcontro

2、lagentsofTrichodermaspp.YangHetong1,2,3,4,TangWenhua2,MaartenRyder3,LiJishun1,GuoYong1,ZhouHongzi1(1BiologyResearchInstituteofShandongAcademyofSciences,Jinan,250014;2Dept.PlantPathology,ChinaAgriculturalUniversity,Beijing,China,100094;3AdelaideLab,CSIROLandandWater,GlenOsmond,SA5064,AdelaideAustra

3、lia;4LifeScienceCollege,ShandongUniversityofTechnology,Zibo,255049)Abstract:Inthisarticle,lectinsof8pathogenstrainswereextractedandpurified.Bytestingagglutinationlectinswith19Trichodermaspp.srains,alllectinsfromfungiagglutinatedtheconidiaofTrichodermaspp.withdifferenttiters,exceptlectinfromAlterna

4、riasolaniMs.ResultindicatedthattheagglutinationofTrichodermaconidiabyfungallectinswasrelatedtotheireffectivenesscausedbythecorrespondingpathogens.StatisticalanalysisshowedsignificantcorrelationbetweentitersofFusariumlectinandtheeffectivenessofTrichodermaagainstthedisease.Theagglutinationtestmightb

5、eappliedtopredictpotentialeffectivenessofagivenTrichodermastrainagainstthecorrespondingdiseaseandthelectinscouldbeemployedinscreeningTrichodermasppasbiocontrolagentsefficiently.Keywords:lectins;pathogens;agglutination;TrichodermasppAsuitablemethodwascrucialforobtainingefficientbiocontrolmicrobiala

6、ntagonistsinsoilborneplantdiseasecontrol.Thestrategiesadoptedbymostresearchersatpresentinscreeningwerevarious(Songetal,1996),butthereweretwobasicones:(1)themostfrequentlyadoptedonewastotesttheinhibitoryeffectincludingantibiosis,mycoparasitismandpathogenlysisofcandidatesonplateandthentesttheefficac

7、yingreenhouseofthemostinhibitorymicroorganisms,followedbyextensiontofieldtrials.Bythisstrategy,anumberofpotentialantagonistswasselectedfromalargenumberofcandidates,andthoseisolateswhichwerelessinhibitoryweredisca

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