资源描述：
《蛋白质相互作用贺俊崎》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、蛋白质的相互作用及其研究方法贺俊崎教授Jq_he@ccmu.edu.cn1——生命信息的体现者生命活动的物质基础第一节蛋白质2生命科学的简要回顾31953....Itallstartedwithclamps,wires&mirrorsTheFirstBiologicalRevolution1962....TheCentralDogmawasborn1975....AutomatedDNAsequencingReplicationDNAmRNATranslation(proteinsynthesis)RibosomeTranscriptio
2、n(RNAsynthesis)Protein4人类基因组计划HGP5GREATEXPECTATIONS26thJUNE2000Thefullysequencedhumangenome:"Withoutadoubtthisisthemostimportantandmostwondrousmapeverproducedbymankind.Thiswillsymbolisethestartofaneweraofmedicaltreatmentanddiagnosiswhichwillhaveaprofoundeffectonhumankindf
3、orcenturiestocome".6Proteinsareatthecenterofactioninbiologicalprocesses.proteinsMotion(myosin,actin,etc)Materialtransport(hemoglobin,transferrin)Metabolism(enzymes)Defence(antibodies,toxins)Mechanicalsupport(collagen)Replicationandrepairingofgeneticinformation(DNAandRNApo
4、lymerases)7Proteins,theexecutantofthelifeprocesses,participateinallaspectsoflife.8Proteomics FromOneRevolutiontotheNext9第二节从基因组计划到蛋白质组学10蛋白质组1994年澳大利亚的MarcWilkins和KeithWilliams首先提出了蛋白质组（Proteome）的概念，早期定义为：微生物基因组表达的整套蛋白质，在多细胞微生物中，整套蛋白质指一种组织或细胞表达的蛋白质。后来定义为：一个基因组所表达的蛋白质。现在蛋白
5、质组的概念为：在一种细胞内存在的全部蛋白质。1112Genome:35,000genesTranscriptome:40-100,000mRNAsProteome:100-400,000proteinsInteractome>1,000,000Genomicsvs.Post-Genomics13Proteinsrarelyworkalone!Protein-proteininteractionsareintrinsictovirtuallyeverycellularprocess(e.g.,DNAreplication,transcrip
6、tion,translation,splicing,secretion,cellcyclecontrol,signaltransduction,orintermediarymetabolism).14细胞凋亡信号是通过蛋白-蛋白相互作用而传递的。死亡受体15Interactionsintheyeastproteome16Cells:theperformacestageofprotein-proteininteractions!17EffectsofProtein-proteininteractionsAlterthekineticprop
7、ertiesofproteins(e.g.,PCNA-DNApolymeased).Resultinformationofnewbindingsite(e.g.,inF1-ATPase,yeasthexokinase,phosphofructokinase).Inactivateaprotein(e.g.,trypsininhibitor-trypsin).Changethespecificityofanenzyme(lactalbumin-galactosyltransferase).18Majorscientificissuesrel
8、atedtostudiesforProtein-ProteininteractionsIdentificationofinteractingpartners(homo-orheteroolig
