Dye-enhanced visualization of rat whiskers for behavioral studies.[2017][eLife][10.7554eLife.25290]

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1、1Dye-enhancedvisualizationofratwhiskersforbehavioralstudies21*1+1+1113JacopoRigosa,AlessandroLucantonio,GiovanniNoselli,ArashFassihi,ErikZorzin,FabrizioManzino,11*4FrancescaPulecchi,MathewDiamond*5Correspondenceto:jrigosa@sissa.it,diamond@sissa.it+6Equallycontributed17International

2、SchoolforAdvancedStudies(SISSA),34136Trieste,Italy89SUMMARY10Visualizationandtrackingofthefacialwhiskersisrequiredinanincreasingnumberofrodent11studies.Thoughmanyapproacheshavebeenemployed,onlyhigh-speedvideographyhas12provenadequateformeasuringwhiskermotionanddeformationduringinte

3、ractionwithan13object.However,whiskervisualizationandtrackingischallengingformultiplereasons,14primaryamongthemthelowcontrastofthewhiskeragainstitsbackground.Herewe15demonstrateafluorescentdyemethodsuitableforvisualizationofoneormoreratwhiskers.16Theprocessmakesthedyedwhisker(s)eas

4、ilyvisibleagainstadarkbackground.Thecoloring17doesnotinfluencethebehavioralperformanceofratstrainedonavibrissalvibrotactile18discriminationtask,nordoesitaffectthewhiskers’mechanicalproperties.19INTRODUCTION20Manynocturnalorcrepuscularrodents,includingratsandmice,havelongvibrissaeth

5、at21enabletactileexplorationofthenearbyenvironment(Grant2009;CarvellandSimons1990;22GustafsonandFelbain-Keramidas1977;Vincent1912).Ratscontactandpalpateobjectsby23projectingtheirfacialvibrissaeinfrontofandaroundthesnout,givingrisetoneuronal24representationsofwhatisbeingcontactedand

6、whereitislocated(Diamond2008,Diamond25andArabzadeh2013).2627Arichvarietyofwhiskerfolliclemechanoreceptors(Ebara2002)encodewhiskerdeflections28withremarkablesensitivityandtemporalresolution(Bale2015,Whiteley2015;Ikeda2014).29Signalsfollowafferentpathwaystoreachtheprimarysomatosensor

7、y,secondary30somatosensoryandtheprimarymotorcortex(Ahissar2000,Kleinfeld2006,Yu2015).31Quantifyingtherelationshipbetweenanimal’ssensingoftheenvironmentandneuronalfiring32inthesesubcorticalandcorticalstagesrequiresprecisemonitoringofwhiskershapeand33motionduringbehavioraltasks.3435T

8、heliteratureputsforwardvar