吴茱萸不同提取物对铜绿假单胞菌的体外抑菌作用研究

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1、吴茱萸不同提取物对铜绿假单胞菌的体外抑菌作用研究作者：乔海霞张彦霞李艳鹏甄攀张玉妥【摘要】目的：研究吴茱萸乙醚提取物、正丁醇提取物、挥发油、水提物对绿脓杆菌的抑菌作用。方法：用乙醚脱脂，得乙醚提取物;将乙醚脱脂后的吴茱萸用70%乙醇提取，正丁醇萃取，得正丁醇提取物1、2、3;用挥发油提取器提取挥发油;用蒸馏水加热回流法提取水提物。用以上提取物分别进行抑菌试验。测定吴茱萸正丁醇提取物1、2中的总皂苷、总黄酮的含量。结果：试验表明正丁醇提取物1、2、3和残渣均在31.25mg/mL时对临床绿脓杆菌仍有抑菌作

2、用;挥发油在1.25%时对临床绿脓杆菌仍有抑菌作用;乙醚提取物对标准绿脓杆菌的MIC为250mg/mL，对临床绿脓杆菌的MIC为125mg/mL;水提物对标准绿脓杆菌无抑制作用，对临床绿脓杆菌的MIC为125mg/mL。正丁醇提取物1、2的总皂苷含量测定结果为28.71%、31.27%;总黄酮的含量分别为43.25%、44.31%。结论：研究提示正丁醇提取物1、2、3和残渣及挥发油对绿脓杆菌有明显的抑菌作用，四者之间的抑菌作用没有显著性区别。水提物和乙醚提取物对绿脓杆菌有抑制作用，抑菌作用比正丁醇提取物

3、、残渣和挥发油的作用弱。10【关键词】吴茱萸;1-丁醇;假单胞菌/铜绿;乙醚提取物;正丁醇提取物;挥发油;水提物;微生物敏感性试验【ABSTRACT】Objective:Tostudytheantibacterialfunctionofrutaecarpaetherextract,n-butanolextract,volatileoilandaqueousextract.Methods:Ethyletherextractwasobtainedbyskimmingether,andrutaecarpade

4、fattedwithetherwasextratedby70%ethanol,thenn-butanolextracts1,2,3weregot;volatileoilwascollectedwithvolatile-oilextractor,andwaterextractwasabstratedbydistilledwaterheatingrefluxextractionmethods.Allaboveextractswereputintostudiesofantimicrobialstudies.F

5、otalsaponins,andflavonoidscontentsinrutaecarpa1,2n-butanolextractweredetermined.Results:Theexperimentsshowedthatthen-butanolextract1,2,3,anditsresiduesin31,25mg/mLwerestillbacteriostasisforclinicalPseudomonasaeruginosaand1.25percentofvolatileoilhadanti-b

6、acteriafurctionforclinicalPseudomonasaeruginosa;theextractrateforethertoMICofstandardPseudomonasaeruginosawas250mg/mL,toMICofchinicalPseudomonasaeruginosawas125mg/mL;waterextracthadnoeffectivenesstoMICofstandardPseudomonasaeruginosaandtoMICofclinicalPseu

7、domonasaeruginosaat125mg/mL.Thetotalsaponinscontentsdetermined10in1,2n-butanolextractwere28.71%,31.27%;andthetotalcontentsofflavonoidswere43.25%,44.31%respectively.Conclusion:Theexperimentsshowthat1,2,3n-butanolextractanditsresiduesandvolatileoilhadappar

8、entantibacteriafunctiontoPseudomonasaeruginosasignificantly,andamongthefourextracts,thereisnosignificantdifferenceinBacteriostasis.WaterextractandetherextracthavebacteriostasistoPseudomonasaeruginosa,butthefunctionisweaker