Tiam 1与PCNA在胃癌中的表达及其临床意义.doc

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1、Tiam1与PCNA在胃癌中的表达及其临床意义【摘要】目的探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)和增殖细胞核抗原(PCNA)在胃癌中的表达及其与临床病理参数的关系。方法采用免疫组织化学法对60例胃癌组织及其相应正常胃黏膜组织标本进行检测。结果①Tiam1蛋白在正常胃黏膜组织中为阴性染色，但在胃癌组织中则呈阳性染色(47/60，78.33%)，两者差异有统计学意义(P<0.05)；②胃癌组织中PCNA蛋白标记指数(PCNALI)(57.49±14.82)显著高于正常胃黏膜组织中的PCNALI(20.74±6.13)(P<0.05)；③Ti

2、am1、PCNA蛋白表达均与胃癌组织分化程度、浸润深度、TNM分期及淋巴结转移显著相关(P<0.05)，但与胃癌患者的性别、年龄及癌灶原发部位、大小无关(P>0.05)；④Tiam1阳性表达胃癌组织中的PCNALI值显著高于Tiam1阴性表达者(P<0.05)。结论Tiam1和PCNA表达上调均与胃癌侵袭转移密切相关，对其进行联合检测有助于判断胃癌的生物学特性。【关键词】胃癌T淋巴瘤侵袭转移诱导因子1增殖细胞核抗原免疫组织化学ExpressionsofTiam1andproliferatingcellnuclearantigeninga

3、striccancerandtheirclinicalsignificanceZHUJinming,YUPeiwu,ZHAOYongliang.DepartmentofGeneralSurgery,GeneralHospitalofJinanMilitaryCommand,Jinan250031,China【Abstract】ObjectiveToobserveexpressionofTlymphomainvasionandmetastasisinducingfactor1(Tiam1)andproliferatingcellnuclearanti

4、gen(PCNA)ingastriccancerandinvestigatetheirrelationshipwithclinicopathologicalparameters.MethodsTheexpressionsofTiam1andPCNAincancertissuesandnormalgastricmucosaof60caseswithgastriccancerweredetectedbyimmunohistochemistrymethod.Results①StainingofTiam1proteinwasnegativeinnormalgas

5、tricmucosabutpositiveingastriccancer(47/60,78.33%),withsignificantlystatisticaldifference(P<0.05).②PCNAlabelingindex(LI)ingastriccancerwas57.49±14.82thatwassignificantlyhigherthan20.74±6.13innormalgastricmucosa(P<0.05).③BothexpressionsofTiam1andPCNAwereassociatedwiththediff

6、erentiationdegree,invasivedepth,TNMstageandlymphnodemetastasisofgastriccancer(P>0.05)butnotwithsexandageofcasesaswellasprimarypathologicalsiteorsizeofthecancerfoci(P>0.05).④PCNALIincaseswithpositivestainingofTiam1wassignificantlyhigherthanthatincaseswithnegativestainingofTi

7、am1(P<0.05).ConclusionsUpregulatedexpressionsofTiam1andPCNAarecloselycorrelatedwiththeinvasionandmetastasisofgastriccancer.CombineddetectionofTiam1andPCNAishelpfulforestimatingthebiologicalbehaviorof5gastriccancer.【Keywords】Gastriccancer;Tlymphomainvasionandmetastasisinducing

8、factor1;Proliferatingcellnuclearantigen;