eae大鼠脊髓中cxcl16、inos的表达情况及依达拉奉的干预作用

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1、TheexpressionofCXCL16、iNOSinEAErats’spinalcordandtheeffectofEdaravoneAbstractObjectiveToobservetheexpressionofCXCchemokineligand16(CXCL16)andinduciblenitrooxidesynthase(iNOS)inthespinalcordoftheexperimentalautoimmuneencephalomyelitis(EAE)ratsmodel,andstudytheinterventionmechani

2、smofedaravoneinEAEandtheeffectofCXCL16andiNOS.Soastoprovidenewideaforclinicaltreatmentofmultiplesclerosis(MS).Methods72femaleWistarratsweredividedintofourgroupsbyrandomizationmethod:normalgroup(Ngroup),EAEgroup,low-dosegroup(LDEgroup),high-dosegroup(HDEgroup).Subcutaneousinject

3、theantigenwhichincludecompleteFreund’sadjuvant(CFA)andguineapigspinalcordhomogenate(GPSCH)per0.5mlinduceEAEmodelinrats.Afterimmunization,eachratinlow-dosegroupinjectedaravoneinjection3mg/kg·ddaily,eachratinhigh-dosegroupinjectedaravoneinjection10mg/kg·ddailyandrecordtheneurolog

4、icalscoreofalltherateveryday.Eachgroupkill6ratsonthe7thday、14thday、21stday.TakethelumbarenlargementofspinalcordforHEstainingandimmunohistochemicalstaining.Observetheinflammatorycells,expressionofCXCL16,expressionofiNOSinthespinalcord.ResultsThere’snoillratinnormalgroup.Theincid

5、encerateofEAEgroupwas77.78%.Eitherthelow-dosegroup’sincidencerateorthehigh-dosegroup’sincidenceratewassignificantlylowerthantheEAEgroup’s（P＜0.05）.TheneurologicalscoreofLDEgroupandHDEgroupwerelowerthanEAEgroup（P＜0.05）,theneurologicalscoreofHDEwaslessthanLDEgroup（P＜0.05）.Theinfla

6、mmatorycells,thepositiveexpressionofCXCL16andiNOSwerefoundinEAE、LDEandHDEgroup,and4万方数据consistentwiththeseverityofdisease.Inthe14thday,thenumberofinflammatorycells、CXCL16-positivecells、iNOS-positivecellsintheLDE、HDEgroupweresignificantlylessthantheEAEgroup（P＜0.05）,andeveryindex

7、ofHDEgroupwaslessthanLDEgroup（P＜0.05）.Inthe21stday,thenumberofinflammatorycells、CXCL16-positivecells、iNOS-positivecellsintheHDEgroupwassignificantlylessthantheEAEgroup（P＜0.05）.Conclusion1.TheCXCL16andiNOSexpressintheEAEratsrelatedwithdiseaseseverity.2.Theinterventionofedaravone

8、canreducetheincidenceofEAErats,alsocanimprovetheclinic